An Association Study Between AKT1 Gene SNP And Major Depressive Disorder

Objective To investigate the association between AKT1 gene SNP and major depressive disorder.Methods All of the subjects were recruited from the Han population in Chongqing.taking the method of a case-control study (case-control study), into a clear diagnosis of 127 cases of patients with MDD and 100 cases of healthy adults. and taking the method of conventional phenol - chloroform extraction of whole blood genomic DNA samples, then choosing the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method ,Analysing AKT1 gene 5 single nucleotide polymorphism( SNP) sites between the two groups in the gene frequency, genotype frequency , haplotype and linkage disequilibrium test (linkage disequilibrium test, LDT) .Result (1) AKT1 gene SNP3 (rs3730358) C allele frequencies for the 92%, T allele frequency was 8% in MDDAND C allele frequency of 97%, T allele frequency of 3 % in the control group , the differences between the two groups was significant (p=0.024),MDD group SNP3 genotype frequency (C/C=84%,C/T =16%), the control group (C/C=94%,C/T=6%), the differences between the two groups are statistically significance (p = 0.020). No difference between the two groups of the gene frequency and genotype frequency of the remaining four SNP loci.(2) linkage disequilibrium test (LDT) results show that, SNP3/SNP4/SNP5 close linkage (D' for the 0.965~0.997), SNP1/SNP2/SNP3 moderate linkage (D' for 0.622 ~ 0.720). haplotype analysis of these sets of SNP loci showed that, CGG haplotyp(eχ~2 = 5.592, p = 0.018, OR = 0.634,95% CI = 0.434 ~ 0.926) and the TGG haplotype (χ~2 = 4.712, p = 0.029, OR = 2.901,95% CI = 1.065 ~ 7.907) of the SNP3/SNP4/SNP5,There were difference between the MDD group and the control group.ATC haplotype(χ2 = 4.707, p = 0.030, OR = 6.216,95% CI = 0.949 ~ 40.714)of the SNP1/SNP2/SNP3,the two groups also have significant differencesConclusion (1) AKT1 gene SNP3 (rs3730358) with MDD is associated(2) AKT1 gene SNP1/SNP2/SNP3. and SNP3/SNP4/SNP5 linkage disequilibrium (3) Haplotype of Certain AKT1 gene with MDD is associated(4) AKT1 gene could be a susceptibility gene of theMDD...