Studies On Anti-tumor And Affecting Immunecells Activities Of Eosinophil

Objective:Eosinophils(eosinophil,EOS)derived from the bone marrow hematopoietic stem cells.In addition to parasitic infection,allergic and skin diseases, eosinophils count increase was found in a variety of tumors and blood diseases.In this study,we investigated the anti-tumor activity and immunoloregulation effect of eosinophils on tumor-bearing mice in vivo.Methods:Leukemia K562 cells and Human Stomach Cancer SGC-7901 cells were treated with EOS which extracted from blood and peritoneal fluid of Sensitized mice at different concentrations for 12h-48h.The proliferation of Leukemia K562 cells and human stomach cancer SGC-7901 were evaluated by MTT assay and growth curve assay.Apply the method of subcutaneous in the right armpit of mice to make the animal model of S₁₈₀sarcoma-bearing mice.After 24h,the mice were randomly divided into 4 groups:the blank control Group,the negative control Group,the positive control Group,the positive control Groupand the eosinopil treatment Group. Observed the growing situation of the tumor tissue in tumor-bearing mice.The mice were killed and separated to tumor tissue,to weight the tumor tissue and assay the inhibitory rate of tumor;Observed the proliferation of mice spleen Lymphocytes in vivo with the synergies stimulation of ConA and LPS and calculated the stimulate index(SI).Observed the morphology of tumor cells by HE(Hematoxylin & Eosin) Staining.And the serum consistence of IL-4,TNF-a were tested with radioimmunoassay.Results: 1.Human Stomach Cancer SGC-7901 cells and eosinopil extracted from the blood of Sensitized mice were cultured in 48 h,showed no obvious effect.(P＞0.05).Leukemia K562 cells and EOS extracted from blood and peritoneal fluid were cultured,showed vary degrees of inhibition after 36 h(P＜0.05);the tumor inhibition rates of blood EOS and peritoneal fluid EOS were 19%and 22%.2.EOS could inhibit tumor tissue growth in S₁₈₀tumor-bearing mice,the tumor inhibition rate was16%.3.With ConA synergies stimulation,the proliferation response capability of T lymphocyte was significantly enhanced(P＜0.05)on EOS treatment Group of mice. The serum consistence of IL-4(0.69±0.32)and TNF-a(1.36±0.36)in EOS treatment Group were significantly higher level different from negative control group (P＜0.05).Conclusions:1.Blood EOS and peritoneal fluid EOS were cultured with leukemia K562 cells,both of the EOS could inhibit the proliferation of tumor cell.Especially cultured with blood EOS,the proliferation of leukemia K562 cells is inhibited more obviously.2.EOS could remarkably inhibit tumor tissue growth in S₁₈₀tumor-bearing mice. The study indicated that EOS could promote the proliferation response capability of T lymphoid and elevate the serum contains of IL-4 and TNF-a,and it's immunoregulation function was regulating activity in S₁₈₀sarcoma-bearing mice.