The Effects Of Female Sex Hormones On Primary Upper Urinary Tract Stone Formation

Objective: To investigate the effects of female sex hormones on urinary stone formation by using an ethylene glycol (EG) and vitamin D-induced rat urolithiasis model and comparing the blood concentrations of female sex hormones between urolithic female patients and controlsMethods : To examine serum concentrations of E2. Prog. PRL. FSH. LH of 36 urolithiasis and 20 normal control females and to compare the results of the two groups.Adult female wistar rats were fed the same diet for four weeks and then were divided into five groups (N=10 each). one group was administered 0.5ml of olive oil three times per week for four weeks as a control. The other four groups were administered 0.5ml of 5% EG and subcutaneously injected 1000Iμvitamin D3 three times per week for four weeks. The rats in three of these four groups were Oophorectomized and the rats of the remaining group underwent a sham operation on the day before the start of the four-week treatment period. Two of the three Oophorectomized groups were administered supplementation of female sex hormones. One was administeredestrogen and progesterone (0.1 mg of estrogen and 2.5mg of progesterone 3 times per week for four weeks) The other was only administered estrogen (o.1 mg of estrogen 3 times per week for four weeks). On the first day of the fifth week of the experimental period, all rats were killed after 24 hours urine of all rats were collected. Then the degrees of crystal deposition were determined histologically and the calcium content in renal tissue, serum calcium and urinary calcium were measured.Results: 1, the crystal deposition and calcium content in renal tissue were greater in the oophorectomized rats compared with the controls, and the same parameters were inhibited by the female sex hormones supplementation. The difference of parameters in two supplement female sex hormone groups was not significant. 2,The difference of serum E2 and Prog between urolithic patients and controls was not significant, too. But serum FSH.LH.PRL of female urolithic patients is higher than controls'.Conclusions: 1,Female sex hormones can inhibit renal crystal deposition in EG-treated rats. Moreover, estrogen plays an important role.2, The effects of female sex hormones on mankind's primary upper tract urinary stone formation need furtherinvastigation...