| Dairy calf diarrhea,which has high incidence and high mortality, is one of major diseases to affect sustainable development of the industry. Huangbai Liquid was used to treat calves humid heat diarrhea with a good clinical effect, which was developed by diagnosis and treatment and reasonable combination according to the theory of traditional Chinese veterinary medicine, modern veterinary science research and study on dairy calf diarrhea's etiology, pathogenesis, pathology, diagnosis and treatment. The prescription of Huangbai Liquid consist of rhizoma coptidis, chinese bulbul, honeysuckle, radix scutellariae, Sanguisorba carbon, smoked plum and the root of kudzu vine, which has the function of clearing heat, depriving the evil wetness, cooling blood to stop diarrhea, antidarrhea with astringent, invigorating spleen and stomach.On the premise of reliable effect in clinic, in order to establish the quality standard of the preparation, to clear the pharmacodynamic mechanism, and to complete the preclinical pharmaceutical research, The orthogonal experiment was used to optimize production process. The thin-layer chromatography was applied to identify rhizoma coptidis, honeysuckle, radix scutellariae, Sanguisorba carbon. And we established the content determination method of berberine by HPLC. The pharmacodynamic mechanism was clarified from the aspects of anti-inflammatory, analgesic, antidiarrheal and enhancement of immune function. The results were as the following.(1) The orthogonal experiment was used to optimize production process. The content of Berberine Hydrochloride as index, the quantity of water in the decocting process was studied by L9(34) orthogonal test. the best preparation process of the preparation was that the drug was boiled for 1.5 hours in 14 times of water for 3 times.(2) The herbs were identified and all the herbs were in accordance with the requirement of the prescription. The method of thin layer chromatography to identify qualitatively the Coptis, honeysuckle, Scutellaria , Sanguisorba carbon was applied. The experimental results have shown that the TLC spots are very clear, specificity, repeatability in the preparation. the method of HPLC to determine Berberine Hydrochloride of coptis as the principal drug was also applied and the content-limited was determined. According to the methodology analyses, the results showed that the stability ,the rate of recovery, reproducibility and accuracy were satisfactory.(3) Huangbai liquid could significantly inhibit the carbon promotion in the small intestine (p<0.05) and significantly reduce the number of diarrhea in mice caused by senna (p<0.05). These indicated that the drug had the function of antidarrhea with astringent. The drug also could significantly inhibit ear edema induced by xylene in mice (p<0.01) and these showed strong anti-inflammatory effect. The drug could significantly reduce the writhing times of mice induced by acetic acid (p<0.01) and these explained its analgesic effect. In the immunity test, the peritoneal macrophage phagocytosis ratio of the mice with splenic asthenia could be significantly increased (p<0.01), and IFN-γand IL-2 levels in serum could also be increased and these experiment reflected that Huangbai liquid could improve the immune function of mice with splenic asthenia.Through the above research, the best preparation process of the preparation was that the drug was boiled for 1.5 hours in 14 times of water for 3 times; The quality of the preparation could be controlled by qualitative identification of Coptis, honeysuckle, Scutellaria , Sanguisorba carbon and content determination of Berberine Hydrochloride. The Huangbai liquid has antidiarrheal function which mechanism are antidarrhea with astringent, anti-inflammatory, analgesic and enhancement of immune function.. |
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