Study On The Distill Technics Of The PMSG From The Endometrium Of Pregnant Mare And Purify The PMSG From Plasma Of Pregnant Mare

Pregnant Mare Serum Gonadotropin(PMSG,or Equine Chorionic Gonadotropin,eCG), which is found in the serum of pregnant mare. In fact ,PMSG is a glycoprotein hormone excreted from the hole of endometrium, molecular weight about 53000.It has the function of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH).It accelerates the growth and maturation of the follicle,and one of its activities,which is similar to the luteinizing hormone (LH), leads to the ovulation of follicle.Presently, PMSG ofen made from the healthy and pregnant mare in the industrialized produce. In order to find the new method of distill,we investigate the PMSG distill technics from uterus of pregnant mare and compare the titer which in the endometrium of pregnant mare and pregnant mare blood plasmaExperiments one This Experiments investigate the content, titer of PMSG which distill from endometrium of pregnant mare.We scrape off mucous membrane surround the calathiform cell from a uterus which is 60 days of pregnancy mare and distill PMSG use existing technique of distill protein.In this course,we discuss the effect of acidity, temperature and metaphosphoric acid .The result indicate that the titer of dialysate from endometrium of pregnant mare is 2800IU/ml, ,the temperature, acidity are also affect the effect of ditill.Experiments two This Experiments investigate the titer of pregnant mare serum.We check the register of hybridization in order to confirm the pregnant days.The result indicate that the titer of pregnant mare serum is different and the titer of pregnant mare serum began to rise from 45 days of pregnancy and the titer of pregnant mare serum reach peak value from 60 days to 90 days of pregnancy.After 90 days of pregnancy, the titer of pregnant mare serum began to decline untill disappear from pregnant mare serum.Experiments Three This Experiments mensurate the titer of dialysate from endometrium of pregnant mare and result show the titer of dialysate ranging between 1700IU/ml and 2800IU/ml. This test select a uterus which is 66 days of pregnancy mare and mensurate the titer of dialysate from endometrium which show that the titer of PMSG is 2800IU/ml,so this test widening the range of choice that the PMSG distill material bring to tremendous economic benefit.