Studies For The Effects Of Puerarin On Mammogenesis

Puerarin, which is one kind of monomer, is a main active component of Pueraria Isoflavone from Kudzu Vine group plants in Pulse Family and its chemical name is 4/, 7-dioxy-8-β-D-gluco-aldehyde Isoflavone. The experiment carried out researches about the effects of three kinds of dosages Puerarin physic liquor on body weights, Mammary gland indexes, Uterus and Ovaries indexes, Progesterone contents, Destradiol contents, Growth hormone contents, Prolactin contents in pedo-sex female mice so as to approach the effects of Puerarin on Mammogenesis of normal pedo-sex female mice. Meanwhile, it provides evidences of scientific theory and experimental references for opening up new ways in increasing lactating cow milk production and also for exploiting new medicines to healthes of humankind and animals. The experimental results showed that:1. Increased body weights value of mice in middle dosage(10mg·kg-1)Puerarin medication administration team, videlicet test group 2 raised a little more than control group's. Low dosage(5mg·kg-1)and high dosage(15mg·kg-1)Puerarin medication administration team, videlicet test group1 and test group 3's degraded a little one by one. The disparation between all test groups and control group is quiet (P>0.05).The presentation of results is middle dosage Puerarin physic liquor has some promotion effect on increasing pedo-sex female mice body weights.2. Mammary gland tissue weights of mice in test group1 and 2 increased one by one a little more than control group's. Test group 3's degraded a little. The disparation between all test groups and control group is quiet (P>0.05). The presentation of results is low and middle dosage Puerarin physic liquors have different extent promotion effect on pedo-sex female mice Mammary gland tissue weights.3. Mammary gland circumscription area of mice in test group 2 increased a little more than control group's. Test group 1's degraded a little. The disparation between test group1and 2 and control group is quiet (P>0.05). Test group 3's degraded obviously, the disparation is extremely significan(tP<0.01). Regarding to Mammary gland parenchyma area of mice, test group1 and 2's increased one by one a little more than control group's but the disparation is quiet (P>0.05). Test group 3's degraded fairly obvious, the disparation is significant(P<0.05).The presentation of results is middle dosage Puerarin physic liquor has some fairly stable promotion effect on pedo-sex female mice Mammary gland circumscription and parenchyma weights.4. Mammary gland ductus transection amount of mice in test group1 and 2 increased by turns fairly obvious more than control group's, the disparation is significan(tP<0.05). Test group 3's increased a little, the disparation is quiet (P>0.05). The presentation of results is every dosage Puerarin physic liquor has different extent promotion effect on pedo-sex female mice Mammary gland ductus branches development.5. Uterus and Ovaries tissue weights of mice in test group 2 increased fairly obvious more than control group's, the disparation is significant(P<0.05).Test group3 and 1's increased a little by turns, the disparation is quiet (P>0.05). The presentation of results is every dosage Puerarin physic liquor has different extent promotion effect on pedo-sex female mice Uterus and Ovaries tissue weights.6. Endometria glandular organ amount of mice in test group 2 increased obviously more than control group's, the disparation is extremely significant(P<0.01). Test group 1's increased fairly obvious, the disparation is significant(P<0.05). Test group 3's increased a little, the disparation is quiet (P>0.05). The presentation of results is every dosage Puerarin physic liquor has different extent promotion effect on pedo-sex female mice Endometria glandular organ amount.7. Ovaries egg follicle total amount and vesicula graafianae amount of mice in test group3, 1 and 2 increased by turns obviously more than control group's, the disparation is extremely significant(P<0.01). The disparation between all test groups is significant(P<0.05). The presentation of results is every dosage Puerarin physic liquor has different extent promotion effect on pedo-sex female mice Ovaries egg follicle development.8. Serum Progesterone contents of mice in test group 2 increased a little more than control group's. Test group3 and 1's degraded a little one by one. The disparation is quiet (P>0.05). The presentation of results is middle dosage Puerarin physic liquor has some promotion effect on pedo-sex female mice serum Progesterone contents.9. Serum Destradiol contents of mice in test group1 increased a little more than control group's. Test group2 and 3's degraded a little one by one. The disparation is quiet (P>0.05). The presentation of results is low dosage Puerarin physic liquor has some promotion effect on pedo-sex female mice serum Destradiol contents.10. Serum Growth hormone contents of mice in test group1, 2 and 3 increased by turns obviously more than control group's. The disparation is extremely significant(P<0.01). The presentation of results is every dosage Puerarin physic liquor has different extent promotion effect on pedo-sex female mice serum Growth hormone contents.11. Serum Prolactin contents of mice in test group1 and 2 increased one by one a little more than control group's. Test group 3's degraded a little. The disparation is quiet (P>0.05). The presentation of results is low and middle dosage Puerarin physic liquors have some promotion effect on pedo-sex female mice serum Prolactin contents.These main conclusions below are yielded from this topic study:1. Middle dosage Puerarin shows some extent phytoestrogen hormonelike promotion effect not only on body weights, Uterus and Ovaries, serum Progesterone contents, serum Destradiol contents, serum Growth hormone contents, serum Prolactin contents but also on Mammogenesis of pedo-sex female mice.2. The dosage-effect dependability of Puerarin is also demanded to confirm further. In this experiment, the effect of Puerarin on mice all experimental indexes generally showed a style of dosage-effect dependability like throwing thing upwards. But, it does not hold a style all in the experimental indexes.3. The effects of Puerarin on the developments of body weights, Uterus and Ovaries, Mammary gland in pedo-sex female mice is identical with their effects on serum Progesterone contents, serum Destradiol contents, serum Growth hormone contents, serum Prolactin contents in the same mice basically on the whole.4. Optimization dosage Puerarin has some extent phytoestrogen hormonelike promotion effect on Mammogenesis of pedo-sex female mice. And its mechanism is possibly as below: 1) regulates the hormone level and synergistic effect of Destradiol, Progesterone, Growth hormone and Prolactin in organism. 2) directly affects on Destradiol receptor of Mammary gland, and displays the biological effect of Estrogen hormonelike. 3) increases the Estrogen receptor amount and affinity of Mammary gland cells, pituitary appendage, hypothalamus endochylema and the Progesterone receptor amount and affinity of Mammary gland cells. 4) elevates the secreting of Ovarian hormone and promotes further on the development of its target organ which is Mammary gland.