Study On The Interspecific Hybridization Compatibility Of Prunus And Tissue Culture In Vitro Of Crossbreeds

The research was carried out from 2005 to 2006 in zhengzhou fruit institute Chinese academy of agriculture sciences and Henna Agriculture University. Eleven Varieties of stone fruits, including peach, apricot and pulm, were used as experimental materials. In order to explore the cross-incompatibility of distant hybridization, 17 cross combinations were designed of prunus. Cross-incompatibility was conquered through mail parents pollen radiated by ⁶⁰Coγ.The factors that affected embryo culture, tissre culture in vitro were studied.Embryo rescue, proliferation, rootage and transplantation of the crossbreeds obtained from distant hybridization were finished. The main resluts were as follows:1. The results obtained from obverse cross and inverse cross among peach, pulm and apricot indicated that different female parents affected cross-incompatibility of distant hybridization significantly. Fruit-setting ratio was 46.11% when peach was used as female parents, much higher than that of apricot and pulm which were 13.66% and 1.6%, respectively. Dfifferent species of peach also affected cross-incompatibility of distant hybridization.2. The pollen germination percentages of katy, Zhouxingshantao and Friar dropped by 50Gy, 150Gy and 300Gy ⁶⁰Coγ radiated.The radiated katy and Zhouxingshantao pollen as male parents,the distant hybridization setting percentage lowered in 2005.whereas, fruit-setting ratio was enhanced by a radiation intensity of 20Gy ⁶⁰Coγ in 2006.3. The resluts from twice surveys showed that most premature fruit dropped had become serious in 30-35d in conbinations in which peach was used as female parents. On the contrary, the conbinations used apricot and pulm as female parents weren't serious. Most of the hybrid used apricot as female parents failed to bear, so embryo culture was...