The Genetic Analysis Of Gynogenetic F₁ Of Transgenic HU-IFN-α Gene Grass Carp

Grass carp is one of our country's major aquatic raised varieties, but it has low survive rate and vulnerability to diseases. The hemorrhagic virus is of the most serious threat, of which costs the whole industry of fresh water cultivation hundreds of millions yuans annually, So the research of disease-resistant breeding for grass carp has continuously being the very emphasis for the aquatic scale. In 1998, 1999, by adopting the technology of molecule recombination, our subject team successfully cloned the coding sequence of HU-a-IFN gene under the control carp β -actin gene promoter. To build up the HU-a -IFN recombinat gene which is able to expressed in the fish body. Then, by the use of the micro- injection technology, we injected the reorganized HU-IFN-a gene into the grass carps' fertilized eggs at its 1-2 cell period to cultivate the trans-HU-IFN-α gene grass carp. Experiment shows that the newly cultivated grass carp has an improved character of resistance the hemorrhage virus, 66% higher than that of the control grass carp. But now we can't transplanted the gene to the point we expected with the present technology of gene transfer. The transplanted gene will be lost when they are heredity and expression on fish's F₁ generation. So establish a population which can stable heredity and expression is our research direction. In order to further studies on the transplanted gene of which its heredity and expression on fish's F₁ generation, and establish a population which can stable heredity and expression. This research aimed to gain the F₁ population of transgenic HU-a-IFN gene disease-resistant grass carp by gynogenic artifical induced. Then analysis the heredity and expression of HU-a-IFN gene in the F₁ population of transgenic disease-resistant grass carp by gynogenic artifical induced. And by the application of the method of artifical sex control and the technology of molecule marker to finish the building of pure F₁ population of transgenic HU-a-IFN gene disease-resistant grass carp by gynogenic artifical induced. This article will center on...