| In order to make it clear salt-tolerance mechanism of Lycium barbarum L. and succession law of between salty environment and Lycium barbarum L, the Lycium barbarum L. that cultivated in Jing-tai County, Gansu and Zhong-ning County, Ning-xia was studied. The physiological indexes of leaves and salt ions content of different organs as well as soil salt content in their root region were measured, microstructure of stem and leaves on Lycium barbarum L. was observed. The study will play very important role in extensively cultivating Lycium barbarum L. in west salinization land and developing west salinization land resource. The main results were as following:1. With the increase of soil soluble salty content, K+, Cl- content and Na+/K+ of Lycium barbarum L. decreased. Under salt stress K+ was firstly transformed to young organs, but Na+ and Cl- for old organs, especially for old leaves. Na+ and Cl- content in old leaves were higher than theirs in young leaves and other young organs. The order of average Na+/K+ ratio among organs of Lycium barbarum L. cultivated in different salty soil was as following: leaves (3.146) >root (1.264) > young fruits(0.267)>young stem(0.223); leaves of two-year-old branch (4.020) >leaves of three-year-old branch (3.840) >leaves of one-year-old branch (1.578). SA value that shows absorption ratio of K+ and Na+ was higher than RS value that presents transportation ratio of K+ and Na+.2. Soluble sugar and proline are important for osmosis, however there were difference between old leaves and young leaves, the soluble sugar content in old leaves was higher than that in young, however the proline content of old leaves was lower than young leaves'. With increase of Lycium barbarum L. years old, the proline content of young and old leaves have low-high-low trend, but the soluble sugar content became higher and higher. The proline content of leaves on one-year-old and two-year-old branch was respectively 0.09ug/g.FW and 0.08ug/g.FW, which were higher leaves on three years old branch than 49.8% and 14.2%. The soluble sugar content of leaves on one-year-old and two-year-old branch was respectively lower than three-year-old branch's 40.4% and 28.8%.3. Membrane permeability and MDA content of Lycium barbarum L. leaves increased under higher soil salt stress. When Lycium barbarum L. branch become older, membrane permeability and MDA content of their leaves all decreased under low soil salt condition, but in higher soil salt condition the membrane permeability decreased, the MDA content increased. When years old of Lycium barbarum become older, their leaves' Membrane permeability and MDA content firstly increased, following decreased.4. Branch years old increasing, Chla,Chlb and Car content of Lycium barbarum L leaves all decreased, however when years old of Lycium barbarum L were increasing, their leaves' Chla,Chlb and Car content firstly increased, following decreased.5. The Na+, K+content and Na+/K+ of leaves which were washed by dd H2O were lower than those that were not washed, EC (electric conductivity) of the used dd H2O was higher than that of dd H2O. With increase of soil salt content, the moisture content of Lycium barbarum L fruits increased, but their sugar content decreased. The relationship between moisture content of leaves (Y) and soil salt content (X) can be showed by the following equation: Y =15.492X+78.219 (R2=0.988 ,n=9)6. When years old of Lycium barbarum L increased, the palisade tissue against underneath epidermis had succession trend toward spongy tissue under salt stress. There were plenty of crystalliferous cells and tannin idioblastas around vascular bundle of mesophyll, in collenchymas inside epidermis as well as parenchyma of pith.7. In 0~100cm soil layer, the average soluble salt content in 30cm,60cm,90cm distance from Lycium barbarum L. trunk decreased respectively 21.5%,23.2%,33.1%, compared with the control. The average soluble salt content in 10~60cm decreased most obviously. In Lycium barbarum L. root areas, K+,Na+,Cl-,Na++K+ /Ca2+... |
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