A Study Of Simple Laboratory Model Of Extracorporeal Membrane Oxygenation (ECMO) And Comparison Of Different Oxygenated Methods

Objective To establish a simple model of ECMO with polypropylene of hemodialysis machine and investigate the effect and possibility of hydrogen peroxide (H₂O₂) as a oxygen supplied method.Methods There were 4 groups in our research. ECMO models were divided into 3 groups on the method of oxygen supplied method: oxygen group, 0.15% H₂O₂ group and 0.06% H₂O₂ group. The original blood was the control group. Using fresh healthy chicken blood pass through ECMO , red blood cell count(RBC), hemoglobin(HGB), hematocrit (HCT), mean corpuscular volume (MCV) ,mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), hydrogen exponent (PH) , partial pressure of oxygen (PO₂) , partial pressure of carbon dioxide(PCO₂), O₂ saturation (SO₂), concentration of bicarbonate (HCO₃^-), total carbon dioxide content (C_CO2) were measured before and after 3 min of ECMO and compared between groups.Results The ECMO model was set up successfully. PH of all 3 groups were significantly raised after ECMO than before ECMO (P=0. 000, P<0.001) . PCO₂ was significantly decreased in 0.06% H₂O₂ group after ECMO than before ECMO (P=0. 000, P<0.001), the other two was also obviously decreased( P=0. 001,0.002, P<0.01) . RBC, HCT and HGB had no significant changes after ECMO in 0.15%...