Create A Transgenic Silkworm Contain Apriona Germari Cellulase Gene(AgEGaseI)

PiggyBac transgenic insect vectors as efficient gene transfer vector in the operational aspects of genetically modified insects have been widely used. The purpose of this study is the use of more sophisticated transgenic technologies such as biological engineering, will exogenous cellulose into the silkworm genome, and then generate the expression of exogenous cellulose can be stable passage of the silkworm. The genetically engineered silkworm can use cellulose as a carbon source, which greatly increase its utilization of mulberry leaves, in sericulture to achieve a major breakthrough.Conclusion:1. By searching germari cellulase gene, complete length cDNA cloning of cellulase, and then build PiggyBac transposon vector, and by microinjection method useful transposon into silkworm eggs.2. By observing the development of eggs and larvae of the eye and neural tube of the fluorescent color to identify the success of GM, using activity assay of exogenous gene in transgenic silkworm AgEGaseI in expression situation.3. The PiggyBac carrier 3 x P3 promoter-induced expression of green fluorescent silkworm eggs and young of the nervous system, the expression of green fluorescence marks the transgenic vector has been successfully imported the silkworm genome, and with integration taken place. Identified by comparison of genomic PCR proved in transgenic silkworm after treatment with gene AgEGaseI.4. Use of different tissue extracts for determination of cellulase activity using AgEGaseI the role of the CMC substrate reaction that generates the glucose in the transgenic silkworm does contain cellulose.