Study On Plasma Membrane Protein Of Roots And Dissoluble Protein In Leaves Of Paulownia In Steady-state Mineral Nutrition Under Water Stress

The theory and techniques for steady-state mineral nutrition of plant, which is relative to the traditional mineral nutrition, is one of view in full new and experimental technique little short of being perfect .The main point of steady-state mineral nutrition can be summarized as follow: (1)According to the requirements of plants to supply the mineral nutrients; (2)Supplying the nutrients in a low concentration which is close to the concentration of the natural soil solution; (3)Supplying the nutrients in a balanced proportion according to the requirements of plants. In this paper, we grow the paulownia with the theory of steady-state mineral nutrition and technology; separate the plasma membrane of roots cell with aqueous two-phase partitioning; determine the enzyme activities of H+-ATPase, EDTA-Fe3+, Fe(CN)63- in roots cell plasma membrane and SOD, POD in plant leaves. Comparing with the plant growing normal nutrition, we find the optimum growth of plants tested have been obtained so that the growth potential of plants has been taped fully. Under water stress by 10% PEG-6000 for 24 hours, the enzyme activities are changed by different degree. The activities of H+-ATPase are decrease in steady-state mineral nutrition and normal nutrition, however the decrease degree in steady-state mineral nutrition is few than that in normal nutrition. This shows that the plants in steady-state mineral nutrition also grow better than these in normal nutrition under water stress. The activities of SOD and POD in steady-state mineral nutrition are higher than these in normal nutrition, under water stress the increasing are also.To further understand the response mechanism of paulownia in steady-state mineral nutrition under water stress, two-dimensional gel electrophoresis(2-DE) is employed to identify differentially displayed protein in leaves. The gels are analyzed with Compugen Z3 image software. 6 spots are up-regulated, 4 spots are down-regulated, 10 spots are differential expression compared with that in steady-state mineral nutrition.