Font Size: a A A

Analysis Of SP1 Binding Region Methylation Associated With Gene Expression In Genome-wide Of Human

Posted on:2011-11-01Degree:MasterType:Thesis
Country:ChinaCandidate:Y FengFull Text:PDF
GTID:2120330338480881Subject:Genetics
Abstract/Summary:PDF Full Text Request
DNA Methylation is a common Epigenatic Phenomenon, and also one of the hot spots of Epigenatic Research. DNA Methylation is a phenonenoun that the transformation of the cytosine within CpG dinucleotide into 5-methyl-Cytosine by DNA methyl-transferase (DNMTs). DNA methylation may repress the expression of related genes.Sp1 is one member of the Sp family. Sp1 has a zinc finger region near its C-end ,which can specially recoginze GC Box on the DNA sequence, and takes part in transcription regulation in mutiply tissues. Sp1 has CpG sites in its binding area on DNA strain, and could be methylated by DNA transferase. Since Sp1 takes part in regulations of many genes, it turns out to be a valuable question that what effect could DNA methylation at the binding site of Sp1 to related genes.This study acts at a global level of whole genome, combines the Sp1 binding site data from UCSC TFBS Conserved track, and methylation data of chromosome 6, 20, and 22 from the Human Epigenome Project, and by applying naive bayes classification method a model for predicting Sp1 methylation status of whole genome-wide was constructed. Then by using this model we predicted and analyzed the methylation status of 1750 Sp1 TFBSs from two tissues (CD4+ lymphocytes, liver cells), and found that Sp1 TFBSs in CpG Island are less liable to be methylated comparing to those Sp1 TFBSs outside the CpG Island. In the same tissue, the methylation status of Sp1 TFBSs are assosiated with gene expression, and genes with a methylated Sp1 TFBS tend to express at a lower level. However, the general expression level of genes near Sp1 TFBSs are not of much difference. In function, the genes near Sp1 binding site and their methylation status do not have notable bias on gene functions.
Keywords/Search Tags:DNA methylation, Sp1, bioinformatics, Genome-Wide Analysis
PDF Full Text Request
Related items