Correlative Basic Research Of Sunflower (Helianthus Annuus L.) Germpalsm Resources

Sunflower (Helianthus annuus L.), come from the North American, was domesticated artificially to be a kind of ornamental, officinal, confectionary and oil plant resources with high economic value. Traditional breeding, radiation breeding and other modern biotechnology had been employed to exploit and protect sunflower germplasm recources, which accelerated the breeding of new sunflower variety. As a new radiation resource, low energy ion implantation was more and more used for sunflower breeding. In addition, seed aging was a prominent problem in germplasm protection engineering. Sunflower seed was the main pool of nutrients and contained abundance of oil, fatty acids and vitamin E etc. Elevating vitamin E content in sunflower seed could prolong the preservative time and improve the economic value of sunflower. In this research, we employed proteomic and physiological methods to analysis proteome changes of N+ implanted sunflower seeds, the mechanism of sunflower aging and the dynamics of vitamin E during seed development.To better understand changes of total protein irradiated with N+ ion further, a proteomic analysis of N+ ion implantation seeds was developed. Two proteins, probably correlated with ion implantation, were identified. The one coverage between the one (No.29) and MADS-box transcriptional factor HAM59 was 23.48% and the other was 23.20% between the other (No.279) and homeobox-leucine zipper protein HAHB-4. Proteome changes induced by N+ implantation were analyzed in this experiment which provided new evidence to study mutation mechanism of ion implantation primarily. Based on those primary results, the mutational"hotspot"about DNA and protein induced by low-energy ion implantation were discussed.Accelerated aging induced by days of exposure to high temperature and high seed water content brings more biochemical deterioration to seeds than usual aging. On the other hand, the cool storage environment and low seed water content under the long-term storage conditions may likely bring the seeds to be in the glassy state. The...