| Purpose:To compare the expression levels of PD-1 and cytokines in patients with Sjogren’s syndrome of different traditional Chinese medicine(TCM)syndromes and healthy people,and to explore the clinical significance of PD-1 and cytokines changes in different TCM syndromes.Material and method:A total of 127 patients with Sjogren’s syndrome who came to the Department of Rheumatology and Immunology of the First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from September 2021 to July 2022 were selected.The general information and blood samples of the patients were collected,and the dialectical classification was performed according to the general information.Flow cytometry was used to measure the expression levels of PD-1 and cytokines in peripheral blood of patients.The differences of PD-1 and cytokines levels in patients with different TCM syndromes were analyzed,and the correlation between PD-1 and cytokines in patients was analyzed.Results:1.Among 127 patients with Sjogren’s syndrome,there were 121 females and 6 males,with a male to female ratio of 1:20.1.In terms of syndrome types,qi and Yin deficiency(34.6%)>liver and kidney Yin deficiency(28.4%)> Yin deficiency and blood stasis(22.8%)> Yin deficiency and heat toxicity(7.9%)> dryness of pathogens attacking the lung(6.3%).2.The PD-1 expression level of pSS patients with Yin deficiency and blood stasis syndrome and Yin deficiency and heat toxin syndrome was higher than that of HC(P< 0.05);The expression level of PD-1 in patients with Yin deficiency and heat toxicity syndrome and Yin deficiency and blood stasis syndrome was higher than that in patients with qi and Yin deficiency syndrome,liver and kidney Yin deficiency syndrome,dry pathogenic factor attack lung syndrome(P< 0.05).3.The mean levels of IL-6,TNF-α and IL-17 in all syndrome groups were higher than the normal value,and the yin-deficiency heat-toxin syndrome group was significantly higher than the other syndrome groups(P< 0.05);There was no significant difference in the levels of IL-2,IL-4,IFN-γ and IL-10 among the three syndrome types(P>0.05),and all of them were in the normal range.4.There were no significant differences in the mean levels of IgA,IgG and IgM among the three syndrome types(P>0.05).There were no significant differences in the positive rates of anti-SSA antibody,anti-SSB antibody and ANA among the three syndrome types(P>0.05).5.The ESR of each syndrome type was higher than the normal value,among which the Yin deficiency and blood stasis syndrome was the highest,followed by the Yin deficiency and heat toxicity syndrome,and these two syndrome types were significantly higher than the qi and Yin deficiency syndrome,liver and kidney Yin deficiency syndrome,dry factor attacking lung syndrome(P<0.05),.CRP was the highest in Yin deficiency and heat toxicity syndrome,followed by Yin deficiency and blood stasis syndrome,which was significantly higher than liver and kidney Yin deficiency syndrome,qi and Yin deficiency syndrome and dry factor attacking lung syndrome(P< 0.05);The syndrome of dryness of pathogens attacking lung was significantly higher than that of liver-kidney Yin deficiency and qi-yin deficiency(P<0.05).6.The expression of PD-1 was positively correlated with IL-6,TNF-α,IL-17 and ESR(P<0.05).Conclusion:1.This disease is more common in women,and the syndrome of qi and Yin deficiency,liver and kidney Yin deficiency,and Yin deficiency and blood stasis are more common;2.The level of PD-1 in pSS patients is abnormal,and PD-1 helps to differentiate Yin deficiency heat and toxin syndrome and Yin deficiency blood stasis syndrome;3.The levels of IL-6,IL-17 and TNF-α in pSS patients are abnormal,which help to differentiate Yin deficiency and heat toxin syndrome.The levels of IL-2,IL-4,IL-10 and INF-γ were normal;4.ESR and CRP can help to differentiate Yin deficiency heat and toxin syndrome and Yin deficiency blood stasis syndrome;5.PD-1 can evaluate the level of inflammation in pSS patients. |
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