Study On The Protective Effect And Mechanism Of Yinchen Siling Decoction On Intestinal Mucosal Barrier In Rats With Alcoholic Liver Injur

Objective:To investigate the effect of Yinchensiling Soup on intestinal barrier function of rats with alcoholic liver disease.Methods:50 male SD rates were divided into the normal group and the experimental group according to the random number table method.The experimental group was fed with high-fat diet and 56 degrees of liquor while the normal group was fed with ordinary diet and distilled water for12 weeks.When the model were established,the successful model rats were randomly divided into3 groups（model group,bicyclol group and Yinchensiling Soup group）.Treated them with corresponding drugs for 4 weeks.The rats’liver function[aspartate aminotransferase（AST）and alanine aminotransferase（ALT）],blood lipid[serum total cholesterol（TC）and triacylglycerol（TG）],endotoxin,inflammatory factor[tumornecrosisfactor（TNF-α）,interleukin-10（IL-10）and Leukotriene B4（LTB₄）],liver histopathology,levels of Occludin and ZO-1,s Ig A（secretory immunoglobulin A）and p Ig R（polymeric immunoglobulin receptor）of each group were measured after drug intervention.Results:1.Compared with the normal group,the biochemical indexess（ALT,AST,TC,TG）,endotoxin,TNF-αand LTB₄ in model group were increased（P<0.01）,the expression of Occludin,ZO-1,s Ig A and p Ig R were decreased（P<0.01）,and IL-10 were decreased（P<0.01）,too.2.Compared with the model group,the biochemical indexes（ALT,AST,TC,TG）,endotoxin,TNF-αand LTB₄ in bicyclol group and Yinchensiling Soup group were decreased（P<0.05 or P<0.01）,the expression of Occludin,ZO-1,s Ig A and p Ig R were increased（P<0.01）,and IL-10 were increased（P<0.01）,too.Conclusion:Yinchensiling Soup can increase the expression of ZO-1,occluding,s Ig A and p Ig R in intestinal mucosa barrier,reduce the endotoxins and inflammatory reaction,reduce liver lipid accumulation and improve liver function.