Based On The "Xuanfu Theory", The Effect Of Acorus Tatarinowii Granules On AQP4 Expression And Brain Edema In Rats With Cerebral Hemorrhage Was Explore

Objective: To study the effect of Shichangpu Peifangkeli on the expression of aquaporin 4(AQP4)and brain edema in rats with cerebral hemorrhage.Methods: A total of 180 SD rats were selected,and 45 were randomly selected as the sham operation group.The remaining 135 rats were prepared according to the method of Gary A.Rosenberg to establish intracerebral hemorrhage(ICH)model.After successful modeling,the rats were randomly divided into ICH model group,SCP26mg/kg group and SCP78mg/kg group,with 45 rats in each of ICH model group,SCP26mg/kg group and SCP78mg/kg group.The sham operation group,ICH model group,SCP26mg/kg group and SCP78mg/kg group were divided into three subgroups: 6h,3d and 7d,with 15 rats in each subgroup.After successful modeling for 6h,the rats in the SCP26mg/kg group and SCP78mg/kg group were given SCP solution by gavage.Gavage twice a day,and the volume of each gavage is 2ml.Rats in sham operation group and ICH model group were gavaged with sterile 0.9% sodium chloride injection of the same volume instead of drug solution at the same time point.Neurological deficits were assessed by longa score,brain edema was observed by brain water content measurement,AQP4 protein level was detected by Western blot,and AQP4 protein expression was localized and quantitatively analyzed by immunohistochemistry.Results:1.Compared with the sham operation group,the ICH model group,the SCP26mg/kg group,and the SCP78mg/kg group showed a trend of increasing neural function defect scores,brain water content,and the protein expression level of AQP4 in the brain tissue of rats starting at6 h,peaking at 3d,and gradually decreasing at 7d;There were no significant differences in6-hour neurological deficit scores,brain water content,and AQP4 protein expression levels in the ICH model group,SCP26mg/kg group,and SCP78mg/kg group(P>0.05);Both the SCP26mg/kg group and the SCP78mg/kg group increased the neurological deficit score,brain water content,and protein expression level of AQP4 in the rat brain tissue at 3 days(P<0.05),while the SCP26mg/kg group increased the neurological deficit score,brain water content,and protein expression level of AQP4 in the rat brain tissue at 7 days(P<0.05).The SCP78mg/kg group all increased the neurological deficit score at 7 days(P>0.05),and the brain water content increased(P<0.05).2.Compared with the ICH model group,there was no significant difference between the SCP26mg/kg group and the SCP78mg/kg group in 6-hour neurological deficit score,brain water content,and the expression level of AQP4 protein in rat brain tissue(P>0.05);Both the SCP26mg/kg group and the SCP78mg/kg group had decreased neurological deficit scores and brain water content on the 3rd and 7th days(P<0.05),especially in the SCP78mg/kg group.The protein expression level of AQP4 in the brain tissue of the rats in the SCP26mg/kg group increased on the 3rd day(P>0.05),the protein expression level of AQP4 in the brain tissue of the rats in the SCP78mg/kg group significantly increased(P<0.05),and the protein expression level of AQP4 in the brain tissue of the rats in the SCP26mg/kg group slightly decreased on the 7th day(P>0.05),The protein expression level of AQP4 in the brain tissue of SCP78mg/kg group increased(P>0.05).3.Compared with the SCP26mg/kg group,the scores of neurological deficits in the SCP78mg/kg group decreased(P>0.05),the brain water content decreased(P<0.05),and the protein expression level of AQP4 in the rat tissues significantly increased(P<0.05);In the SCP 78mg/kg group,the neurological function score decreased(P<0.05),the brain water content decreased(P<0.05),and the protein expression level of AQP4 in rat tissues increased(P>0.05).Conclusion: Shichangpu Peifangkeli can promote AQP4 protein expression in rats with intracerebral hemorrhage,reduce brain edema and improve neurological deficits in a dose-dependent manner,and its mechanism may be related to promoting AQP4 protein expression.