Exploring The Effect Of “Red Peony Root-Aconite Powder” On Hepatocyte Inflammatory Injury Based On PI3K/Akt Signaling Pathwa

Objective: Exploring the effect of “radix paeoniae rubra-radix aconiti lateralis” on inflammatory hepatocytes based on the phosphoinositide 3-kinase(PI3K)/protein kinase B(PKB,also known as Akt)signaling pathway.Methods: The L02 cell line was cultured in vitro,divided into the normal group,the model group,the blank plasma group,medicated plasma(radix paeoniae rubra-radix aconiti lateralis) group,and the Copanlisib(PI3K inhibitor)group,then interventing accordingly.Screening intervention concentration and time of medicated plasma by CCK-8 method;observing the ultrastructure of cells by transmission electron microscope;determining the m RNA and protein expressions of PI3K/Akt signaling pathway by RT-q PCR and Western-blot.Results: Compared with the normal group,modeling increases the number of damaged organelles and autophagosomes,while decreases the expressions of PI3K/Akt m RNA and phosphorylated protein(P<0.01).Compared with the model group,the medicine group were relatively normal in cell morphology and decreased autophagosomes,while increased the m RNA expressions and phosphorylated protein of PI3 K and Akt(P<0.01 or P<0.05).Conclusion: Radix paeoniae rubra and radix aconiti lateralis may play a role in protecting hepatocytes by regulating PI3K/Akt signaling pathway.