Study On The Intervention Effect Of Jiawei Erxian Pill On Bronchial Asthma Model Rats Based On SOCC Pathwa

Objective: To investigate the effect of Jiawei Erxian Pill on calcium ion channel(SOCC)controlled by calcium store in rats with bronchial asthma.Methods: 54 SD rats were divided into blank group and modeling group by random number table method,with 9 in blank group and 45 in modeling group.The rat asthma model was established.After successful modeling,the rats in the modeling group was randomly divided into model group,low dose group of Jiawei Erxian Pill,medium dose group of Jiawei Erxian Pill,high dose group of Jiawei Erxian Pill and Gushen Dingchuan Pill group,with 6 rats in each group.Each drug group was given corresponding drug intervention.Rats in the model group and the blank group were given the same volume of normal saline by gavage,twice a day,for 28 consecutive days.He staining was used to determine whether the asthma model was successfully prepared.The positive expression of STIM1 and ORAI1 was measured by immunohistochemistry,the relative expression of STIM1 and ORAI1 was measured by Western blotting,and the average fluorescence intensity of calcium ions was observed by fluorescence indicator method to reflect the change of calcium ion concentration.Results: In the model group,there were a large number of inflammatory cells infiltrating the bronchial branches and their surroundings,mainly lymphocytes.A small number of inflammatory cells infiltrated in the low,medium and high dose groups of Jiawei Erxian Pill and the Gushen Dingchuan Pill groups.The injury degree of rats in each treatment group was between the blank group and the model group,and there was no significant difference in the injury degree of each treatment group.Compared with the blank group,the average optical density,protein expression and calcium ion average fluorescence intensity of STIM1 and ORAI1 in the lung tissue of rats in the model group were significantly increased,the average optical density,protein expression,ORAI1 protein expression and calcium ion average fluorescence intensity of STIM1 in the lung tissue of rats in the low-dose group of Jiawei Erxian Pill were increased.The difference was statistically significant(P<0.05).Compared with the model group,the average optical density,protein expression and the average fluorescence intensity of calcium ions of STIM1 and ORAI1 in the lung tissue of rats in the high-dose group of Jiawei Erxian Pill were significantly decreased,the expression of STIM1 and ORAI1 in the lung tissue of rats in the Gushen Dingchuan Pill group was decreased,and the expression of ORAI1 protein in the lung tissue of rats in the low and medium dose groups of Jiawei Erxian Pill was decreased,with statistical significance(P<0.05).Conclusion:Jiawei Erxian Pill can inhibit the activation of SOCC in the lung tissue of asthmatic rats,and thus inhibit the influx of calcium ions,which plays a role in the treatment of bronchial asthma.