Analysis Of Alzheimer's Disease Gene Network Based On Network Science And Statistical Method

Alzheimer’s disease(AD)is a chronic age-associated neurodegenerative disorder.The incidence of AD increases exponentially with age.Over the age of 65,4-8% of the elderly may suffer from this disease.AD has become the fourth killer after heart disease,cancer,and stroke.At present,the pathogenesis of AD is not clear,and clinical drugs used commonly can only relieve symptoms within a certain period of time but cannot improve the disease fundamentally.In this paper,AD genes are collected from multiple databases,and the gene network of AD is constructed by considering some factors such as gene co-expression and metabolic relationship.The gene network is divided into modules by Molecular Complex Detection(MCODE)Cluster,Markov Clustering(MCL),Community cluster(Glay)and Cluster one.Then these division methods are evaluated by network structure entropy,and the optimal division method,MCODE.Through functional enrichment analysis,the functional modules are identified.Furthermore,essential genes can be predicted by the analysis of network topology characteristics such as degree centrality(DC),betweenness centrality(BC)and proximity centrality(CC)of these functional modules.In addition,the integrated algorithm(logical regression algorithm under Bayesian framework)is used to predict AD’s essential genes.The final predicted essential genes are obtained by analyzing these two results above.Aiming at the difficult problem that the clinical drugs do not have a better therapeutic effect on AD,the multi-components and multi-targets characteristics of herbs are considered.On the basis of identifying the essential genes and gene modules of AD,based on network pharmacology,four kinds of KXS,DGSYS,YGS,YQTYTactive compounds-active compound targets network and AD common core network are constructed,then the better herbs and herb compounds of AD are selected through the Gene Ontology(GO)enrichment analysis(including Biological Process(BP),Cell Component(CC),Molecular Function(MF))and KEGG pathway enrichment analysis.Theoretical knowledge is provided for the follow-up study of herbs in the treatment of AD,and a feasible scheme is provided for the study of “drug-target-disease”.