| [Objective]To explore the correlation between the expression of human papillomavirus(HPV)and P16 and immune cell markers and prognosis in head and neck squamous cell carcinoma(HNSCC).[Methods]A total of 265 surgical specimens of head and neck squamous cell carcinoma sent for examination by China-Japan Friendship Hospital from January 2016 to April 2022 were retrospectively collected.53 pathologic cases meeting the study criteria were screened out,including 18 cases of nasopharyngeal cancer,15 cases of oropharyngeal cancer,10 cases of tonsil cancer and 25 cases of laryngeal cancer.There were 15 female patients,ranging in age from 29 to 93 years,with a median age of 58 years.HPV virus was detected by HPV and P16,and the infiltration level of immune cells was detected by immunohistochemistry.[Results]By PCR detection,there was no significant difference in gender,age and HPV expression in cyclin in HNSCC and benign cases(P>0.05).In HNSCC cases,there was no significant difference in HPV expression among different age and gender groups(P>0.05),but CD4,CD8,CD20 and CD163 were significantly different between HPV-positive and HPVnegative cases(P<0.05).For P16 detection,there was no significant difference in HPV expression between gender and age groups in HNSCC and benign cases(P>0.05),but there were significant differences in the infiltrating cells of CD4.CD8 and CD56 between P16 positive and P16 negative cases(P<0.05).In HNSCC cases,there was no significant difference in the expression of P16 between different gender groups(P>0.05),but there was significant difference in the expression of P16 between different age groups(P<0.05).CD4,CD20,CD56,CD163 and Foxp3 were not significantly different between P16 positive and P16 negative cases(P>0.05).CD8 was significantly different(P<0.05).In HNSCC cases,the younger the age,the longer the mean survival time.The mean survival time of positive patients was longer than that of negative patients(ranging from more than 4 to 26 months).P16-positive patients had a lower probability of risk of recurrence or death,which was only one-fourth that of patients with negative cancer.[Conclusions]HPV and P16 were different in the detection of immune infiltration.HPV-positive HNSCC patients had a higher degree of immune cell infiltration,a lower risk of recurrence or death,and a better survival than HPV-negative HNSCC patients.CD8 showed a positive effect on survival,while the role of CD4,CD56,CD163 was not clear.[Objective]To investigate the infection and tumorigenicity of human papillomavirus(HPV)in the squamous epithelium of hemorrhoids.[Methods]From 6208 hemorrhoids samples in China Japan Friendship Hospital from May 2015 to May 2020 and diagnosed as hemorrhoids,135 hemorrhoids samples containing typical and atypical hollowed cells in squamous epithelium mucosa were selected and 30 hemorrhoids samples without hollowed cells in squamous epithelium mucosa in the same period were randomly selected as the control group.HPV virus was detected and classified by PCR hybridization,and samples were detected by immunohistochemistry P16.[Results]The cases of hemorrhoids with hollohed-out cells in squamous epithelium accounted for 2.17%(135/6208)of all hemorrhoids.The positive rate of HPV was 71.1%(96/135)and that of P16 was 52.59%(71/135).According to the severity of the overlying squamous epithelial mucosa hyperplasia,the experimental group was divided into mild dysplasia,moderate dysplasia,severe dysplasia/carcinoma in situ,and perianal squamous cell carcinoma.The positive rates of HPV infection in the four groups were77.50%(62/80,single virus infection 74.19%,multiple severe virus infection 25.81%),71.43%(15/21,single virus infection 80%,multiple severe virus infection 20%),42.11%(8/19,single virus infection 87.5%,Multiple-severe HPV accounted for 12.5%),73.33%(11/15,single-type HPV accounted for 90.91%,multiple-severe HPV accounted for 9.09%),among which the infection rates of high-risk HPV were 11.29%(7/62),33.33%(5/15),100%(8/8),90.91%(10/11),respectively.52 cases of single low-risk infection,23 cases of single high-risk infection and 21 cases of mixed subtypes of HPV were detected.[Conclusion]The HPV infection in hemorrhoid squamous epithelium is low,and the hollohed-out cells in the histology is an important indication.Some cases have multiple viral subtype infection.The dysplasia of squamous epithelium is closely related to HPV infection,and the degree of dysplasia is closely related to the expression of HPV subtype and P16.Hemorrhoids with hollowed-out cells should be tested by HPV subtype combined with immunohistochemical P16. |
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