Study On The Characteristics Of Intestinal Flora In Obese Patients With Type 2 Diabetes Mellitus With Damp-heat Obstructing The Spleen Syndrom

Purpose: Using 16 S r DNA gene sequencing technology,the abundance,diversity and colony composition of the intestinal flora of patients with type 2 diabetes mellitus(T2DM)and healthy people were detected,and the structural characteristics of the intestinal flora of patients with obese type 2 diabetes with damp and heat trapped spleen were further explained,and the objective criteria and new ideas for the clinical prevention and treatment of this disease were provided.Material and method:From March 2021 to October 2021,2021,20 cases of endocrinology were diagnosed in the Department of Endocrinology of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,and 20 cases of obese type 2 diabetes mellitus patients with damp and hot spleen syndrome were identified as the observation group,and 20 healthy people matching sex and age matching with the observation group were recruited in Shenyang As the control group,and the 16 S r DNA gene sequencing technology was used to extract dna from fecal samples,and PCR amplification of gene region 16 s V3-V4 was carried out,and a sequence library was constructed Illumina performed hands-on sequencing,and the sequence of samples from the last two groups of people was compared with the abundance,diversity and colony composition analysis of the intestinal flora.Results:1.There was no statistical difference in gender and age between the observation group and the control group(P>0.05),but there was a statistical significance in BMI(P<0.05)2.A total of 948 OTUs(sequence sets)were obtained in this study,and the dilution curve of the species indicated that the sample size of this study was sufficient.3.Diversity analysis results,compared with the control group,Chao1,ACE,Shannon,Simpson index in Alpha diversity decreased,and the difference was statistically significant(P<0.05),indicating that the abundance and diversity decreased.The results of PCo A in Beta diversity indicated that there were differences in the composition of flora between the two groups(P<0.05).4.The results of species composition analysis showed that compared with the control group,the abundance of Proteobacteria increased,while the abundance of Bacteroidetes and Firmicutes decreased in the observation group at the phylum level.At the class level,the abundance of γ-Proteobacteria increased,while the abundance of Clostridium and Bacteroidetes decreased.At the order level,the abundance of Enterobacteriaceae increased,while the abundance of Clostridium and Bacteroidetes decreased.At the family level,the abundance of Enterobacteriaceae increased,while the abundance of Ruminobacteriaceae decreased.At the genus level,Escherichia/Shigella increased.5.The linear discriminant effect(LEf Se)results found that the observation group can be divided into Proteobacteria,γ-Proteobacteria,Enterobacteriaceae,Enterobacteriaceae,Escherichia/Shigella,Klebsiella 6 species of bacteria are used as key biomarkers,and healthy people can be divided into Actinobacteria,Firmicutes,Bacteroidetes,Clostridium,Bacteroidetes,Bacteroidetes,Clostridium,Eight bacterial groups,including Rumenobacteriaceae,were used as key biomarkers.Conclusions:1.The abundance and diversity of intestinal flora in obese patients with type 2 diabetes mellitus with damp-heat trapping the spleen are lower than those in healthy people,and the composition of the bacterial colony has changed.2.Proteobacteria,γ-Proteobacteria,Enterobacteriaceae,Enterobacteriaceae,Escherichia/Shigella,Klebsiella 6 species may be used as damp-heat trapped spleen syndrome obesity 2 Potential key biomarkers in patients with type 2 diabetes mellitus,providing targets for clinical prevention and treatment.