Identification Of The FtsH Gene Family In Tea Plant And Functional Analysis Of The CsFtsH31 Gen

FtsH gene plays an important role in plant stress resistance.In this study,theFtsH gene was identified in the Camellia sinensis genome,and its bioinformatics analysis was carried out.The CsFtsH31 gene was overexpressed in tobacco,and its stress resistance function was analyzed.The results are as follows :1.Identification and analysis of Camellia sinensisFtsH gene family.By comparing with theFtsH genome of Arabidopsis thaliana,45FtsH Camellia sinensis genes were identified in the Camellia sinensis genome,and the 45 Camellia sinensisFtsH genes were unevenly distributed on the Camellia sinensis chromosome.According to the evolutionary relationship,theFtsH gene of Camellia sinensis plant was subdivided into five categories;gene expression analysis showed that CsFtsH gene had a high expression level in leaf tissue,and some genes increased with the increase of leaf maturity;Different CsFtsH genes were differentially expressed under PEG-induced drought stress,salt stress,methyl jasmonate stress and cold treatment.2.Expression analysis of CsFtsH gene under high temperature treatment.Fluorescence quantitative expression analysis of CsFtsH gene in leaves of Fuding Dabaicha under high temperature stress treatment showed that the expression levels of CsFtsH14,CsFtsH31,CsFtsH34 and other genes in leaves increased with time under high temperature stress treatment.And the expression level of CsFtsH31 gene increased the highest,indicating that the CsFtsH gene is induced by temperature and is closely related to high temperature stress.3.Functional verification of CsFtsH31 in transgenic tobacco.The overexpression vector p BWA(V)KS-CsFtsH31-GUS of the Camellia sinensis CsFtsH31 gene was constructed,and the tobacco was genetically transformed to obtain transgenic tobacco.The wild-type and transgenic tobaccos were treated with exogenous light oxidant MV,and it was found that the chlorophyll content of transgenic tobacco was higher than that of wild-type tobacco,the content of malondialdehyde was lower than that of wild-type tobacco,the content of soluble sugar was higher than that of wild-type tobacco,and the content of supernatant was higher than that of wild-type tobacco.The activity of oxide dismutase was higher than that of wild-type tobacco,indicating that transgenic tobacco was more resistant to photooxidation than wild-type tobacco.At the same time,the high temperature resistance ability of transgenic tobacco and wild-type tobacco was also analyzed.Under the high temperature stress of 38 °C,the chlorophyll content of transgenic tobacco was higher than that of wild-type tobacco,the malondialdehyde content of transgenic tobacco was lower than that of wild-type tobacco,and the content of transgenic tobacco was lower than that of wild-type tobacco.The superoxide dismutase activity was significantly higher than that of wild-type tobacco,indicating that the CsFtsH31 gene expressed in tobacco can scavenge the reactive oxygen species produced by stress,maintain the metabolic balance of reactive oxygen species,protect the function of the membrane structure,and improve the resistance of tobacco to high temperature stress.