Discovery Of Leaf Color Regulatory Genes And Molecular Marker Development In Dachang Tea Purple Bud Line P113 Based On Transcriptomic

Tea variety is an important factor to determine the quality of tea.Tea germplasm resources are the material basis for the breeding of new varieties.Purple bud tea is an excellent tea resource with high anthocyanin content.Understanding the genetic mechanism of anthocyanin metabolism regulation of purple bud tea is of great significance to improve the breeding of characteristic purple bud tea varieties and the development of high anthocyanin tea products.Therefore,based on the determination of anthocyanin content and metabolic components of different lines of Camellia tachangensis F.C.Zhang(purple bud line P113 and green bud line P114)and purple bud line P113 in different seasons,this paper studied the genes related to anthocyanin synthesis of purple bud line P113 by transcriptomics.At the same time,based on the analysis of transcriptome data that anthocyanin accumulation is related to the expression level of genes related to anthocyanin synthesis pathway,an In Del marker was performed.The results of this study are as follows:(1)Ten anthocyanin derivatives were detected in the buds and leaves of purple bud strain P113.Among them,cyanidin-3-o-caffeioyl sophoricoside-7-o-glucoside,cyanidin acetyl glucose,delphinium-3-o-glucoside and geranium-3-o-glucoside were up-regulated with seasonal development,which was closely related to the change of leaf color of ’P113’;(2)Four enrichment pathways of differentially expressed genes were obtained by using KEGG database: anthocyanin biosynthesis,phenylalanine metabolism,flavonoid biosynthesis,flavonoid and flavonol biosynthesis;(3)The differentially expressed genes in ’P113’ were HCT(TEA007063,TEA028105),and the differentially expressed transcription factor was myb(TEA029004);A total of 16 differentially expressed structural genes and 14 differentially expressed transcription factors were obtained from the two sets of transcription data;(4)It is speculated that FLS(TEA 010762),HCT(TEA 007063,TEA028105)and transcription factor MYB(TEA028476)are the key bases for the formation of ’P113’bud leaf purple;...