Study On The Correlation Of Intestinal Microbe Between MGMT Methylation And Non-methylated Glioma

Objective This study aims to discuss whether DNA repair protein O~6-methylguanine-DNAmethyltransferase(O~6-methylguanine-DNA Methyltransferase,MGMT)is methylated or not,and the composition and differences of intestinal flora in glioma patients.By revealing the relationship between MGMT methylation and intestinal flora structure,further explore the mechanism of temozolomide resistance,and finally provide new ideas for improving the prognosis of patients with Temozolomide resistance.Methods The patients of the neurosurgery Department of Ningxia Medical University General Hospital from January 2021 to July 2022 were selected as the experimental Group,and the patients whose postoperative test results were glioma were divided into MGMT negative Group and MGMT positive Group according to the results of MGMT test.29 cases were collected in the positive Group,and 24cases were collected in the negative Group.A total of 25 healthy subjects were selected as the control Group.During the same period of time,fecal samples of experimenters in each Group were collected,DNA of various microorganisms was extracted from the fecal samples,and 16Sr DNA V3-V4 fragments in the extracted DNA were amplified,and analyzed by high-throughput sequencing technology.Results High-throughput sequencing results showed that there were significant differences in intestinal flora in phylum,class,order,family and genus between MGMT positive glioma patients and MGMT negative glioma patients as well as healthy people.In general,the relative abundance of intestinal flora in the MGMT-positive Group was higher than that in the MGMT negative Group,which in turn was higher than that in the normal control Group.In the MGMT positive Group,the relative abundance of Firmicutes,Clostridium,and Spirillae was higher than that in the MGMT negative Group,while in the MGMT negative Group,the relative abundance of Bacteroidetes and its subbranches was higher significantly higher than MGMT positive Group.(p<0.05)Conclusion There are significant differences in gut microbiota between patients with and without MGMT methylation.The differences were mainly manifested in low-degree phyla,mainly in the changes of inflammatory flora,such as Clostridium,Streptococcus,and Barnes bacteria.Some of the above microflora may be potential biomarkers for judging MGMT methylation,and the changes of these microflora may affect the therapeutic effect of TMZ on glioma,thus providing new ideas for the treatment of glioma.