Microbial Characterization Of Stage Ⅰ Non-small Cell Lung Cancer And Exploration Of Potential

Objective: To investigate the characterisation of microorganisms in alveolar lavage fluid from patients with stage Ⅰ non-small cell lung cancer and benign lung nodules and to analyse the differences between them to predict their clinical value in lung cancerMethods: Forty patients with stage Ⅰ non-small cell lung cancer and 21 patients with benign lung nodules who met the inclusion criteria from December 2021 to December 2022 at the First Affiliated Hospital of Jiamusi University were collected.Bronchoscopy was performed preoperatively,and stage Ⅰ non-small cell lung cancer and benign lung nodules were confirmed by our pathology department after surgery.Bronchoalveolar lavage fluid specimens were collected from 40 patients with stage Ⅰ non-small cell lung cancer and 21 patients with benign lung nodules.Sixty-one alveolar lavage fluid specimens meeting the above criteria were sequenced by 16 Sr RNA high-throughput sequencing technology,and the microbial expression of the two groups was measured and counted for biostatistical study.Results: 1.The microbial composition of bronchoalveolar lavage fluid from patients with stage Ⅰ non-small cell lung cancer and benign lung nodules was more complex and diverse at the genus level;the microbial alpha diversity results showed that the abundance and homogeneity of microorganisms in the lungs of patients with benign lung nodules were significantly(p<0.05)higher than those of patients with stage Ⅰ non-small cell lung cancer.2.There was a significant difference in bacterial community composition between patients with stage Ⅰ non-small cell lung cancer and patients with benign nodules(p<0.05),with benign nodules having a more dispersed community and stage Ⅰ patients having a single lung flora structure.3.Pseudomonas,Streptococcus spp.and Gracilibacter spp.were significantly(p<0.05)more abundant in the lungs of patients with benign nodules than in patients with stage Ⅰ nonsmall cell lung cancer,whereas Bacillus,Anoxybacillus and Burkholderia spp.species were,in contrast,significantly(p<0.05)enrichment in the lungs of patients with stage Ⅰ non-small cell lung cancer.Conclusions: 1.The microbiological composition of alveolar lavage fluid from patients with stage Ⅰ non-small cell lung cancer differs significantly from that of benign lung nodules,and the microbiological composition may be used as a marker in the assessment of lung cancer.2.Microbial alpha diversity was significantly higher in benign nodules than in stage Ⅰ nonsmall cell lung cancer,and microbial alpha diversity may be used as a marker to assess lung cancer.3.Potentially pathogenic microorganisms of the genus Bacillus are enriched in the lungs of lung cancer patients and may serve as potential microbial markers.