The Expression And Role Of Small Nucleolar RNA Host Gene 20 In Cholangiocarcinoma

Background and PurposeCholangiocarcinoma(CCA)was a recognized malignant cancer that originated from bile duct mucosal cells and accounted for 10% to 15% of hepatobiliary malignant cancers.In recent years,the death rate caused by cholangiocarcinoma was increasing.More than 70% of patients often lacked characteristic symptoms of cholangiocarcinoma in the early stage and had missed the best opportunity for surgical treatment.Long non-coding RNA(lncRNA)was correlated with the malignant biological characteristics of many tumors.Among them,small nucleolar RNA host gene 20(SNHG20),as a long non-coding RNA,had been found to play a key regulatory role in many cancers.However,its biological function and mechanism of action in cholangiocarcinoma had not been clarified.The purpose of this study was to investigate the role of long non-coding RNA SNHG20 in cholangiocarcinoma and its molecular mechanisms in the regulation of proliferation,invasion and migration.Methods(1)The expression level of SNHG20 in CCA patients was detected by TCGA database and qRT-PCR.(2)Combined with the clinical and pathological data of 49 CCA patients,the relationship between the SNHG20 expression in CCA patients and the pathological characteristics of tumor was analyzed.(3)The expression of SNHG20 in CCA cells was detected by qRT-PCR.(4)After regulating SNHG20 expression,the effects of SNHG20 on the proliferation,invasion and migration of CCA cells were investigated.(5)Bioinformatics website and dual luciferase reporter assay were used to predict and verify the targeting binding site of SNHG20 to miR-520f-3p.(6)The expression of miR-520f-3p in CCA tissues and cells was detected.(7)Exogenous reduction of miR-520f-3p,to explore its regulation of CCA cell proliferation,invasion and migration ability.Results(1)SNHG20 expression was higher in cholangiocarcinoma tissues,and the high level of small nucleolar RNA host gene 20 expression in CCA was associated with clinicopathological information of patients: Patients with high expression of SNHG20 were more likely to develop lymphatic invasion and later TNM stage.(2)The growth rate,migration efficiency and invasion ability of cholangiocarcinoma cells transfected with Si-SNHG20 were restricted.(3)Bioinformatics websites predicted the binding sites of SNHG20 and miR-520f-3p,and dual luciferase reporter assay verified the targeted binding of SNHG20 and miR-520f-3p.The expression of miR-520f-3p was significantly down-regulated in CCA tissues and cells,and negatively correlated with the expression of SNHG20.(4)Cell function assays found that overexpression of miR-520f-3p could inhibit the proliferation,invasion and migration of CCA cells.Conclusions(1)LncRNA SNHG20 was highly expressed in CCA tissues.And abnormally high expression of SNHG20 was closely associated with lymphatic invasion and later TNM stage.(2)Regulatory SNHG20 expression could influence the proliferation,migration and invasion ability of CCA cells.(3)SNHG20 expressed at a high level in CCA played a carcinogenic role through adsorption of miR-520f-3p.