Peripheral Blood MicroRNA124-3p,BDNFmRNA And BDNF Expression Levels And Cognitive Function In Schizophrenia

Objectives: 1.To elucidate the targeted regulatory relationships among microRNA124-3p,BDNFmRNA and BDNF;2.To explore the correlation between microRNA124-3p,BDNFmRNA and BDNF expression levels and cognitive function impairment in schizophrenia.Methods: Eighty-nine schizophrenia patients who met the diagnostic criteria of ICD-10 and 72 healthy controls were selected.The clinical symptoms of schizophrenia patients were assessed by PANSS scale.The cognitive function of the subjects was assessed by MCCB cognitive function test tool.The target binding sites of microRNA124-3p and BDNFmRNA were queried in the bioinformatics database.The reverse transcription real-time fluorescence quantitative polymerase chain reaction(RT-q PCR)was used to detect the relative expression of microRNA124-3p and BDNFmRNA in the subjects’ peripheral blood,and enzyme-linked immunoassay(ELISA)was used to detect the concentration of brain-derived neurotrophic factor(BDNF)in the subjects’ peripheral blood.SPSS26.0 software was used to establish the database for statistical analysis.Results: 1.Micro RNA124-3p expression was elevated in the patient group(P<0.001,statistically significant),BDNFmRNA and BDNF expression was decreased(P<0.001,statistically significant),and several aspects of information processing speed,word learning,reasoning and problem solving,and visual learning were significantly decreased in the MCCB compared to the control group(P<0.001,statistically significant).2.There was a significant correlation between the relative expression of microRNA124-3p and BDNF concentration in both the patient and control groups,with a moderate negative correlation in the patient group(P<0.001,statistically significant)and a weak negative correlation in the control group(P<0.05,statistically significant).3.No statistically significant correlations were seen between microRNA124-3p relative expression,BDNF mRNA relative expression,and BDNF concentration and PANSS total score,positive symptom score,and negative symptom score in the patient group.4.Correlation analysis of PANSS total score,positive symptom score,negative symptom score,microRNA124-3p relative expression,BDNF mRNA relative expression and BDNF concentration with MCCB score showed that in the patient group,positive symptom score was positively correlated with RPS-T score(P<0.05,statistically significant),negative symptom score was positively correlated with VIL-T score(P<0.05,statistically significant),microRNA124-3p relative expression was negatively correlated with VEL-T score(P<0.05,statistically significant),microRNA124-3p relative expression was negatively correlated with RPS-T score(P< 0.001,statistically significant),microRNA124-3p relative expression was positively correlated with VIL-T score(P<0.05,statistically significant),BDNF mRNA relative expression was positively correlated with SOP-T score(P<0.05,statistically significant),and BDNF concentration was positively correlated with RPS-T score(P<0.001,statistically significant);in the control group,BDNF concentration was positively correlated with RPS-T score(P<0.001,statistically significant),and the rest did not show statistically significant correlation.Conclusions: 1.Preliminary observations suggest that microRNA124-3p may specifically bind to BDNF mRNA through specific base binding sites,exerting a negative regulatory effect on it and thus interfering with the amount of BDNF transcriptional synthesis;2.Reasoning and problem solving abilities were significantly and positively correlated with BDNF expression levels,so BDNF expression in peripheral blood can contribute to the assessment of the severity of cognitive impairment in schizophrenic patients.