| Background: Hyperlipidemic pancreatitis is characterized by vomiting,abdominal pain,elevated trypsin and dyslipidemia,and the severity of pancreatitis is positively correlated with cholesterol and triglycerides.In severe cases,multiple organ dysfunction,shock,heart failure and death can occur.The etiology and pathogenesis of hyperlipidemic pancreatitis are still unknown.Treatment of HLAP mainly consists of fasting and low-calorie infusion(in acute Settings).The standard treatment is based on fluid resuscitation(intravenous resuscitation),nutritional support,etiological identification,and pain relief accompanied by lipid-lowering drugs.The guidelines report that early,adequate fluid management is the cornerstone of their treatment,but it is mainly for mild patients,and fluid resuscitation does not prevent necrosis,organ failure,and death.Even fluid resuscitation may be harmful in patients with severe acute pancreatitis.With the increasing incidence of HLAP and the severity of complications,it can be seen that the prevention and treatment of HLAP is urgent and extremely important.Current clinical therapy found that combine traditional Chinese and western medicine treatment of hyperlipidemia pancreatitis after the better,and her virginity tiaozhi capsule from the view of the different development of traditional Chinese medicine,found that FTZ of HLAP may have certain prevention function,by adjusting the hub of liver and spleen,blood to turbidity,improve HLAP disorder of liver and spleen,supplemented by treatment caused by diet not festival and hot and humid card each knot of HLAP pixu(spleen deficient);Clinically,high risk patients with dyslipidemia of HLAP usually take lowering blood lipid level as the main target for prevention and treatment.FTZ may play a role in the prevention and treatment of HLAP by improving blood lipid levels.lipid levels.Objective:1.To select the best modeling method of acute pancreatitis model.2.Using emulsion higher fructose to hyperlipidemia model rats,and to their virginity tiaozhi capsule lavage for medicine,with fenofibric for positive medicine,observe the general situation of rats,to detect rats blood lipid after four joint cattle sulfonated cholic acid sodium(hyperlipidemia pancreatitis rat model,and discuss the possible mechanism of intervention for traditional Chinese medicine hyperlipidemia pancreatitis research provides the experimental basis of objective.3.In this study,sodium taurocholate model rats were given Zhenzhu Tiaozhi capsule by gavage and levofloxacin was used as the positive drug to observe the general situation of the rats and evaluate the anti-inflammatory effect of the rats.To explore its possible mechanism and provide an objective experimental basis for the study of TCM intervention on acute pancreatitis.Methods: The model method of acute pancreatitis was established by using urenin and sodium taurocholate to select the suitable model method.Then the hyperlipidemia model was established with high fructose combined with high fat emulsion,and the normal group and the model group were set up,and the hyperlipidemia of the rats was recorded.Two weeks after the model group was established,the hyperlipidemia in the model group was evaluated,and two doses of FTZ-L and FTZ-H,levofloxacin and fenofibrate were given by gavage every afternoon,respectively.Among them,the HLAP group was given by gavage of emulsion and high fructose water every morning.The general performance of the animals was observed during modeling and administration,and lipid levels were measured for two weeks after modeling and four weeks after administration.Four weeks after prophylactic administration,the acute pancreatitis model was established by surgical modeling with 5% sodium taurocholate.The contents of NF-κB and IL-6 and TNF-α in pancreatic tissues of rats were determined by ELISA kit.Pathomorphic changes of pancreas were analyzed by HE staining under light microscopy in part of the pancreas,and gene expression levels of COX-2 and IKK in other parts were determined by q RT-PCR.Results:1.The results of amylase test in the model rats operated with sodium taurocholic acid showed that,compared with the normal group,AMS of NIU was increased and showed more severe acute pancreatitis;Compared with the normal group,AMS was significantly increased in Rhymenin group.There was no significant difference in AMS in the saline group.2.AMS and LPS test results of HLAP model group showed that,compared with AP model group,AMS and LPS of HLAP model group were increased,showing a more serious state of acute pancreatitis;Compared with low dose of FTZ,AMS of HLAP was significantly increased.AMS was significantly reduced in the low-dose FTZ group compared with the fenofibrate group and the high-dose FTZ group.3.The results of four blood lipid tests in the HLAP model experiment showed that,compared with the AP model group,the four blood lipid tests in the HLAP model group were increased except for the decrease of HDL-C,showing the disorder of lipid metabolism,among which TC was significantly increased;HDL-C in HLAP was lower than that in fenofibrate,high-and low-dose FTZ groups.Compared with the fenofibrate group,TC and TG of rats in the low-dose FTZ group were decreased.The results of four blood lipid tests of rats in the HLAP model experiment showed that,compared with the AP model group,the levels of four blood lipids in the HLAP model group were increased except for HDL-C,showing lipid metabolism disorders,among which TC was significantly increased.Compared with the fenofibrate group,the HDL-C of HLAP was decreased in both the high-dose and low-dose FTZ groups.Compared with the fenofibrate group,the TC and TG of the low-dose FTZ group were decreased.LDL-C was significantly lower in the high-dose FTZ group than in the fenofibrate group.Conclusion: Hyperlipidemia can aggravate the inflammatory state of pancreatitis,and the effects may be bi-directional.FTZ may play a role in the prevention and treatment of hyperlipidemic pancreatitis through the NF-κB inflammatory pathway... |
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