| Objective: To observe the correlation of high mobility group protein B-1(HMGB-1)with clinicopathological characteristics,gut microbiota in colorectal cancer(CRC)patientes using bioinformatics database,and to explore whether its influences colorectal cancer prognosis is related to intestinal microflora imbalance.Methods:(1)The clinical datas of GSE12945,GSE17536,and GSE17537 data sets in colorectal cancer from the Gene Expression Omnibus(GEO)database were collected,and HMGB-1 was analyzed by receiver operating characteristic(ROC)curve to obtain a cutoff value of 10.24 ng/m L,and divided into HMGB-1 high expression group(HMGB-1 H)and low expression group(HMGB-1 L),and anlyzed to observe the relationship of HMGB-1 and the clinical features,prognosis of colorectal cancer.(2)Combined with the expression of HMGB-1 in serum samples of88 colorectal cancer patients in our hospital from May 2021 to November2021,and use enzyme linked immunosorbent assay(ELISA)to detect the expression of HMGB-1.According to the Cutoff value of the GEO database,They were divided into HMGB-1 H group(>10.24ng/m L)and HMGB-1 L group(≤10.24ng/m L).10 patients from two groups were selected for 16 S r DNA sequencing to observe and analyze whether HMGB-1 affects the progression of colorectal cancer through changes in intestinal flora.Results:(1)According to the completeness of the data,a total of 232 GEO cases were collected,and the ROC curve was used to analyze the impact of HMGB-1 on the prognosis of CRC.It was found that the area under the curve was 0.587,and the best Cutoff value was 10.24ng/m L,the difference was statistically significant Significant(P<0.05).(2)Compared with HMGB-1 L group,HMGB-1 H group had worse TNM stage(P=0.010)and shorter overall survival(P=0.005)and shorter disease free survival(P=0.035),and was more prone to recurrence and metastasis of colorectal cancer(P=0.006),suggesting that HMGB-1expression was a risk factor of CRC patients(P=0.013),The risk of death in CRC patients with high expression of HMGB-1 was 6.204 times higher than that in patients with low expression.The 5-year overall survival rates of HMGB-1 H group and HMGB-1 L group were 67.9% and 95.6%,respectively.(3)The serum of 88 patients with CRC was collected in this study,and the average level of HMGB-1 was 10.33ng/m L,which was close to the average value of GEO data.(4)The results of 16 S r DNA sequencing showed that regardless of species diversity or species richness,the HMGB-1 L group was significantly better than the HMGB-1 H group,but the HMGB-1 H group was mainly Firmicutes,while the HMGB-1 L group was dominated by Proteobacteria.At the genus level,we found that HMGB-1 L group was mostly enriched in Providencia.The functional prediction of Tax4 Fun showed that the functional enrichment of the two groups was mostly enriched in DNA damage repair function,glycolysis,amino acid metabolism,cell motility and so on.Conclusion:(1)The increase of HMGB-1 is closely related to the poor prognosis of CRC.The CRC death rate of patients with high HMGB-1 is 6.204 times that of patients with low HMGB-1.(2)The diversity and richness of bacterial flora in patients with low HMGB-1 is better than that in patients with high HMGB-1,the reduction in the enrichment of Providencia in patients with high HMGB-1 may affect the prognosis of CRC One of the factors,the mechanism may be related to the DNA damage repair and metabolism of CRC cancer cells. |
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