| In recent years,with the gradual improvement of living standards,the number of people suffering from diabetes has been increasing year by year worldwide.At present,there is no radical cure for diabetes.The diabetes and a series of complications caused by it will greatly affect the life quality of patients,and may threaten the life safety of patients in serious cases.Therefore,accurate and timely detection of blood glucose level is of great significance for the prevention and treatment of diabetes.At present,a variety of detection methods have been applied in the study of glucose detection,among which the electrochemical detection method is widely used in the field of glucose detection because of its advantages of simple equipment,high efficiency and reliable results.Enzyme-based glucose electrochemical sensors have been commercially applied,but it is difficult to achieve a further improvement in the detection level due to the inherent disadvantages of enzymes,while the nonenzymatic electrochemical glucose sensors can avoid the use of enzymes with unstable activity and achieve effective detection of glucose at the same time.Enzyme-free electrochemical glucose sensors still have some disadvantages,such as complex preparation procedure and poor repeatability.Based on this,the nonenzymatic electrochemical glucose sensors was studied in this paper.The research contents and conclusions are as follows:（1）Copper-platinum nanoparticles modified glassy carbon electrode was successfully constructed for electrochemical glucose detection.A one-step in-situ electrodeposition method avoided the complicated modification process and the addition of extra adhesive,and had the advantages of simple,fast,and green.The characterization of the material confirmed that the average nanoparticle size was about 100 nm.The introduction of alloy nanoparticles gave a better play to the advantages of large specific surface area and synergistic effect.The effect of glucose detection was verified by electrochemical behavior evaluation.The results shown that the constructed sensor exhibited a high sensitivity of 2209μA m M-1 cm-2,with a linear detection range of 10～750μM and a limit of detection of 1.8μM（S/N=3）.At the same time,the sensor had good reproducibility and anti-interference ability,and could achieve a certain degree of recovery performance by simple chemical reduction.The results in artificial saliva and real human serum confirmed that the sensor had certain application potential.The ideal performance of glucose detection could be obtained through green and facile electrode modified process.（2）On the basis of the former work,to avoid the use of noble metal which will increase cost and to achieve the goal of light-weight electrode,copper selenide modified self-supporting carbon paper substrate was successfully constructed by electrodeposition followed by chemical vapor deposition and was applied to glucose detection research.Chemical vapor deposition was different from the traditional transition metal chalcogenides synthesis strategy,which further broadened the application field of chemical vapor deposition.Low-cost chemical vapor deposition method also owns large application potential in glucose sensors.The constructed sensor shown excellent performance,with the sensitivity of 5391μA m M-1 cm-2 and 3004μA m M-1 cm-2 in the linear range of 5～200μM and 200～760μM,respectively,and the limit of detection was 1.2μM.It also exhibited good anti-interference ability,reproducibility,the stability of continuous test and long-term storage stability.Meanwhile,the detection results in simulated saliva and real human samples also proved that the constructed sensor owned certain practical application potential. |
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