The Study On The Bidirectional Regulation Effects Of Ultrasound Stimulation On Cortical Neurovascular Coupling In Mice

Ultrasound stimulation(US)is a high-precision neuromodulation technology that can penetrate the skull and has multiple advantages such as selectivity,targeting,reversibility,and non-invasiveness.Many scholars at home and abroad have been studying ultrasound stimulation.Ultrasound visual stimulation is the use of ultrasound to stimulate the eyes of mice,and its effects with different parameters on neural activity and blood oxygenation in the visual cortex of mice are unclear.In addition,studies have shown that transcranial ultrasound stimulation(TUS)is the use of ultrasound through the skull to stimulate the mouse brain,which can modulate cerebral blood oxygenation,neural activity,and neurovascular coupling characteristics in the resting state mice,but the effects in the task state mice on neural activity,cerebral blood oxygenation and neurovascular coupling remains to be elucidated.This thesis mainly studies the effects of ultrasound stimulation on cortical neural activity,cerebral blood oxygenation and neurovascular coupling through the above two ultrasound stimulation methods.Firstly,this thesis introduces the background,status and significance of the relevant research content,and then introduces the experimental device and the principle of the experimental platform.Then,the data processing methods of local field potential(LFP)and cerebral blood oxygenation are described in detail.Subsequently,ultrasound visual stimulation experiments with different parameters were performed.The electroencephalogram acquisition system was used to record the LFP and the optical intrinsic signal imaging system was used to record the cerebral blood oxygenation.The results show that ultrasound visual stimulation with different parameters can elicit responses in the visual cortex of mice.Next,we designed different modes of TUS,used TUS to stimulate mice under peripheral sensory stimulation,and simultaneously recorded LFP and cerebral blood oxygenation data.The results show that TUS,under specific parameters,can modulate the neural activity and cerebral blood oxygenation of mice under peripheral sensory stimulation,and have bidirectional regulation effects on neurovascular coupling.In summary,ultrasound stimulation can regulate the neural activity and cerebral blood oxygenation in mice,and has bidirectional regulation effects on neurovascular coupling.This study opens new research areas for the potential applicability of ultrasound stimulation in brain disorders related to neural activity,cerebral blood oxygenation,and neurovascular coupling.