| Objective: Early after dental implant surgery,intraoral pathogenic bacteria tend to colonize the implant surface and occupy cellular sites,increasing the chance of secondary infection,resulting in delayed osseointegration or decreased quality and consequent implant failure.In this study,we constructed a bifunctional composite coating of micro-arc oxide-coupled HA and heat-permeable Na Cl based on the surface modification of micro-arc titanium oxide,analyzed the material chemistry of the coating,and observed the biological behavior of mouse embryonic osteoblast precursor cells(MC3T3-E1)and Staphylococcus aureus(ATCC25923)on the surface of the specimen.This project provides new ideas and a theoretical basis for the success of osseointegration in the early stage of implantation in patients with missing teeth.Methods:1.In this study,pure titanium specimens were made into 11.5 mm diameter and 0.2 mm thickness discs,and the electrolyte was prepared with calcium acetate,sodium hydroxide,and disodium β-glycerophosphate to prepare micro-arc oxidation coating(MAO)only;hydroxyapatite was added to the electrolyte,and hydroxyapatite(MAO-HA)was loaded on the pure titanium sheets by micro-arc oxidation technique,and then magnetic stirring thermal infiltration was used to Composite sodium chloride coating(MAO-HA-Na Cl).The surface morphology and roughness of the specimens were observed by scanning electron microscope,the hydrophilicity of the specimens was analyzed by contact angle meter,the elemental composition of the specimens was determined by EDS energy spectrometer,and the phase composition of the surface of the specimens was analyzed by X-ray diffractometer for the above four groups of coatings.2.Cell proliferation on the surface of these four groups of specimens was tested by Cell Counting Kit-8 staining.The number of adherent cells on the surface of the material for 24 h was detected by fluorescent staining,stained with live/dead cell stain,observed,and photographed under a fluorescent microscope,and the number of cells was counted using Image-Pro Plus software.3.The cells were co-cultured by bacterial cells for 2,4,and 6h,fixed using 2.5% glutaraldehyde fixative for 4h,and dehydrated with 30%,60%,70%80%,90%,95%,and 100% gradient concentrations of ethanol,ventilated and dried,and observed by scanning electron microscopy after gold spraying on the surface of the specimen.Results:1.In this experiment,the micro-arc oxidation coatings prepared by setting multiple sets of conditions were compared after shooting scanning electron microscopy,and the best parameters were selected as voltage 350 V,frequency 600 Hz,duty cycle 15%,and reaction time 6 min.It was observed under an electron microscope that the coatings prepared under this parameter had good porosity and roughness,the specimens with composite hydroxyapatite were more hydrophilic,and the EDS energy spectrum data analysis showed that HA could be more uniformly distributed throughout the micro-arc oxidized coatings.2.In terms of biocompatibility evaluation,this experiment used CCK-8 and DAPI staining methods to detect the biocompatibility of the specimens and found that the coating of pure titanium surface micro-arc oxidized with hydroxyapatite and sodium chloride had no significant inhibitory effect on cell proliferation and had relatively good cytocompatibility.3.By bacterial cell co-culture and scanning electron microscopy observation,it was found that the coating could guide the cell homing early and reduce the adhesion and colonization of bacteria.Conclusion: The micro-arc titanium oxide surface compounded with hydroxyapatite and sodium chloride has good biocompatibility and antibacterial properties,which can effectively guide cell homing and combat early colonization and secondary infection in a relatively short period time.This composite coating material offers new therapeutic prospects for further development of implant restorative materials to promote osseointegration and anti-infection in the future. |
PDF Full Text Request