Study Of Temporal Heterogeneity Of Lung Cell Carcinogenesis Based On Raman Spectroscopy

Objective:This research aimed to investigate the biomolecular changes in human lung epithelial cells at different times after benzo(a)pyrene induction,and to achieve the observation of temporal heterogeneity of lung cell carcinogenesis based on Raman spectroscopy,and to promote the progress of the study of lung cancer cell heterogeneity.Methods:Human lung epithelial cells were treated with benzo(a)pyrene at a concentration of5μg/m L to characterize the degree of cellular carcinogenesis by morphological changes,proliferation,invasion and tumorigenic capacity.The primary,5th,15 th,25th and 35 th generation toxin-contaminated cells were cultured and smears were prepared.Raman spectral data were then obtained using a spectral scanning test system,and feature extraction was performed by multivariate statistical analysis(principal component analysis-linear discriminant analysis)to analyse the heterogeneity of metabolic molecules at the cell level during different periods of lung cell carcinogenesis.Results:With the increasing number of generations of the toxin-contaminated cells,the morphology of the cells changed significantly,and their proliferation,invasion and tumorigenic abilities increased,which proved that the degree of cell carcinogenesis gradually increased.The average Raman spectra of normal cells and cells from the 5th,15 th,25th and 35 th generations after toxicity contamination were basically similar.By multivariate statistical analysis,nucleic acids,lipids,proteins,carotenoids and glucose were the main differential biomolecules,with a decreasing trend in the content of nucleic acids,lipids,proteins and carotenoids over time,an increasing trend in the content of glucose,and an increasing trend in the ratio of proteins to lipids,proteins to nucleic acids and nucleic acids to lipids,with particularly marked differences after the 15 th generation.Conclusion:Human lung epithelial cells are exposed to air for a long time and are sensitive to external stimulation causing cellular carcinogenesis and temporal heterogeneity with changes in the microenvironment.With the stimulation of lung cells by benzo(a)pyrene,the structure of the genetic material is damaged,leading to nucleic acids instability.At the same time,cellular metabolism is altered,showing lipid peroxidation,disturbances in amino acid metabolism,inhibition of carotenoid secretion and reprogramming of glucose metabolism.