Expression And Clinical Correlation Of MT-CYB In Colorectal Cancer Tissues

Objective:Colorectal cancer(CRC)is one of the most common malignancies of the gastrointestinal tract with a poor clinical prognosis and limited detection options.Mitochondrially encoded cytochrome b(MT-CYB)is a key enzyme in the mitochondrial oxidative respiratory chain and is involved in the transfer of these negatively charged electron particles to drive complex III to produce ATP.It is not clear.In this study,we used immunohistochemistry(IHC)to determine the protein expression of MT-CYB in CRC tissues to clarify the correlation between MT-CYB expression and the clinicopathology and survival prognosis of CRC patients.Methods:This study is divided into two parts.The first part is the bioinformatics analysis of MTCYB single gene,using the relevant transcriptional gene datasets from public databases,and exploring the potential function and significance of MT-CYB in colorectal cancer through analysis of gene differences,analysis of clinical baseline data and COX regression analysis.In the second part,postoperative paraffin blocks and case data of patients who underwent radical colorectal cancer surgery from 2011 to 2014 were collected from the Fifth Clinical School of Shanxi Medical University,and their corresponding cancer and paracancerous tissues were selected and made into tissue microarrays(TMAs).The protein expression of MT-CYB in the tumor tissues and paracancerous tissues of CRC patients was determined by immunohistochemistry,and the correlation between the expression of this gene and the clinical indicators of CRC and postoperative survival was analyzed.Results:MT-CYB was mainly located in the cytoplasm of colorectal cancer tissues,and the expression of MT-CYB in cancerous tissues was significantly higher than that in cancerous tissues,and the difference was statistically significant(P<0.01).MT-CYB may serve as an independent diagnostic factor for colorectal cancer,but there was no significant difference in postoperative survival between patients with high and low MT-CYB expression.In addition,MT-CYB may participate in related immune response by affecting immune response activation cell surface receptor signaling pathway and immune response activation signal transduction and may activate immune response by affecting immune factor differentiation such as Th1,Th2 and Th17.Conclusion:MT-CYB may affect the occurrence and development of colorectal cancer by activating cell surface receptor signal pathway,Rap 1 signal pathway and chemokine signal pathway through immune response.In addition,MT-CYB may serve as a potential biomarker for the diagnosis and immune infiltration of CRC patients.