| Objective: Aging refers to the gradual loss of molecular fidelity after reaching sexual maturity,resulting in loss of function and eventually leading to disease and death.This experiment aims to investigate the anti-liver-aging effect and mechanism of salidroside(Sal)against natural aging C57 mic.Methods:A total of 50 C57 mice were randomly selected from 10 mice reared to 3 months old as the young control group,the other 40 mice were fed to 15 months of age as early aging mice,and were randomly divided into aging group,positive drug cycloastragenol(CAG)group,salidroside low-dose(Sal-L)and salidroside high-dose(Sal-H)group.The mice in young control group and aging group were given the same amount of normal saline10 m L/kg intragastric administration,while the mice in positive drug group was given 20mg/kg of CAG intragastric administration,and the mice in Sal-Land Sal-H groups were given 25 mg/kg and 100 mg/kg of salidroside respectively,once a day for 60 days.After the treatment,the aging degree of mice was scored.Colorimetric method was used to detect the activities of monoamine oxidase(MAO)and superoxide dismutase(SOD)in serum.The contents of lipid peroxide malondialdehyde(MDA)and oxygen free radicals(ROS)in serum were detected by thiobarbituric acid assay and fluorescent probe assay respectively.The morphological structure of mouse liver tissue was observed by hematoxylin-eosin staining.The fibrosis of mouse liver tissue was observed by Masson staining and lipofuscin deposition was observed by aldehyde fuchsin staining.The protein expressions of telomerase reverse transcriptase(TERT)was detected by histochemical assay,,and the experssions of TERT,c-Myc and p-c-Myc were determined by Western Blotting.Results: Compared with the young group,the aging degree score of the aging group mice was higher,the serum MAO activity,MDA content and ROS level were significantly increased,and the serum SOD activity was significantly reduced.The morphology and structure of liver tissue cells were disordered,the cell bodies were atrophied,and liver fibrosis and lipofuscin deposition were aggravated.Immunohistochemical and Western Blot results showed that the protein expression of TERT and p-c-Myc was significantly reduced.After treatment with salidroside,the aging degree score,serum MAO activity,MDA content and ROS level of aging mice decreased significantly,and serum SOD activity increased significantly.Pathological staining showed that salidroside could improve the cellular morphology and structure of liver tissue,reduce liver fibrosis and lipofuscin deposition in aging mice.Immunohistochemical and Western Blot results showed that after treatment with salidroside,the expression of TERT and p-c-Myc in liver tissue of aging mice increased.Conclusion: Salidroside can reduce the aging degree score of aging mice,reduce their oxidative stress damage,improve their cell histomorphology and structure,and delay the aging of mouse hepatocytes by increasing the expression of p-c-Myc and TERT protein in the hepatocytes of aging mice. |
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