| BACKGROUND:Gastric cancer(GC)is one of the most common malignant tumors in the digestive tract worldwide,and its most common histological subtype is adenocarcinoma,accounting for over 95%of gastric cancer cases.In clinical practice,most gastric cancer patients have already experienced metastasis or progression to a higher degree of malignancy upon diagnosis,resulting in poor prognosis.In order to improve the prognosis of gastric cancer patients,there is an urgent need to discover molecular markers for early diagnosis and improvement of the prognosis of gastric cancer patients.Research reports that the aminoacyl tRNA synthetase(ARS)family is a class of proteins with important functions that can bind specific amino acids to the reverse codons on tRNA and participate in biological processes such as cell metabolism,tumorigenesis,innate immunity,and angiogenesis.Tryptophan tRNA synthetase(WARS)belongs to Class I ARS and is expressed by IFN-γ Induced.Previous literature has reported that WARS is expressed in a variety of cancers(ovarian cancer,oral cancer,melanoma,colorectal cancer,etc.)and is related to prognosis.There are few reports on the expression of WARS in gastric cancer and its impact on the prognosis of gastric cancer patients.Objective:The propose of this study was to analyze whether there was any differences in the expression of WARS in gastric cancer tissues and paraneoplastic tissues by detecting the expression of WARS in gastric cancer tissues and paraneoplastic tissues;and further to analyze the correlation between the level of WARS expression and the clinicopathological features of gastric cancer patients and its value in the prognosis of gastric cancer patients.METHODS:1.RT-qPCR and immunohistochemistry were used to detect the mRNA and protein expression levels of WARS in 27 cases of fresh gastric cancer tissues and normal tissues adjacent to the cancer,and the data were compiled and analyzed.2.65 paraffin specimens of gastric cancer treated in the Department of Surgery of the First People’s Hospital of Nanyang City between December 2016 and January 2018 were collected,and the protein expression levels of WARS in 65 gastric cancer tissues were detected using immunohistochemical techniques,and the correlation between the expression of WARS and the clinicopathological parameters and postoperative survival of patients was analyzed with the clinical case data of 65 gastric cancer patients.Results:1.RT-qPCR showed that the expression level of WARSmRNA in gastric cancer tissues was higher than that in paraneoplastic tissues,and the above results were statistically significant(t=2.104,P<0.05).2.Immunohistochemical results showed that among the 27 fresh gastric cancer tissues and paraneoplastic tissues:the high expression rate of WARS in cancer tissues was 70.4%(19/27);the high expression rate of WARS in paraneoplastic tissues was 40.7%(11/27);at the protein level,WARS expression in cancer tissues was higher than that in paraneoplastic tissues,and the results were statistically significant(X2=4.80,P<0.05).3.Immunohistochemical detection results showed that in 65 gastric cancer paraffin specimens:the expression of WARS correlated with the degree of tumor differentiation,and the patients with high WARS expression had low tumor differentiation,and the findings were statistically significant(P<0.05).There was no apparent correlation with patient’s age,gender,tumor size,presence of vascular cancer emboli,depth of tumor infiltration,and lymph node metastasis(P>0.05).4.Cox single-factor regression analysis showed that WARS expression level,lymph node metastasis,and tumor differentiation were factors influencing the prognosis of patients(P<0.05);Cox multi-factor regression analysis failed to show that WARS expression level was an independent risk element for overall survival of gastric adenocarcinoma patients after surgery(P>0.05).5.Applying the Kaplan-Meier method for survival analysis,the median overall survival was 28 months in 65 gastric cancer patients;The overall survival of patients with high WARS expression was longer compared to those with low WARS expression,the difference between the two groups was statistically significant(Log-rank=0.025,P<0.05).Conclusions:1.The expression of WARS in gastric cancer tissues was higher than that in paraneoplastic tissues,and its expression correlated with the degree of tumor differentiation,suggesting that WARS may influence the development of gastric cancer.2.The overall survival of patients with high WARS expression was longer compared to those with low WARS expression in gastric adenocarcinoma tissues,suggesting that gastric cancer patients with high WARS expression have a better prognosis. |
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