Study On State Transtion Of Lung Adenocarcinoma Cells Based On Ensemble Eigen Microstate Theory

Lung cancer is one of the malignant tumors that bring both health and economic pressure to people.The latest cancer statistics in the world has shown that the incidence and mortality of lung cancer are close to the top of the list of cancer incidence and mortality.Lung adenocarcinoma is the most common subtype of lung cancer,accounting for about 40 % of the total number of lung cancers.The study on the occurrence and development mechanism of lung adenocarcinoma is of great significance for its prevention and treatment.A cell is a complex system far from equilibrium.The process of cell evolution from normal state to tumor state is a non-equilibrium process.Using non-equilibrium statistical physics to analyze problems in the life process can not only clarify the dynamic process of cell evolution,but also understand the mechanism of tumorigenesis,and it is a further attempt to apply non-equilibrium statistical physics methods in the field of life sciences.The ensemble eigen microstate theory based on statistical physics theory has been proposed recently,which is not only applicable to equilibrium processes but also non-equilibrium processes.It has been widely used in complex systems such as the Earth’s climate system and stock system.In this paper,based on the transcriptome data and clinical data of lung adenocarcinoma in the The Cancer Genome Atlas(TCGA)database,and the ensemble eigen microstates method is used to study the difference between the states of normal cells and tumor cells and the reasons for the state evolution,and the main conclusions are as follows:(1)The weights of the first three eigen microstates are larger than those of other eigen microstates,so the microstates of normal cells and tumor cells can be regarded as the superposition of the first three eigen microstates,respectively.(2)There are significant differences between the first,second,and third eigen microstates of normal cells and tumor cells,indicating that there is state transition when normal cells evolve into tumor cells.(3)The difference for the microstates of normal cells and tumor cells is mainly in the biological processes involved in intracellular genes: the biological processes that the genes of the new first eigen microstate of normal cells are mainly enriched in are related to the body’s defense work.the biological processes that the genes of the new first eigen microstate of tumor cells are mainly enriched in are related to cell proliferation,and these gene expression products are closely related to the immune escape mechanism of tumor cells.(4)The correlation of normal cells and tumor cells with proto-oncogenes and tumor suppressor genes is different.The correlation of proto-oncogenes in tumor cells is significantly larger and the microstate of the normal cell has a larger relation with tumor suppressor genes.This is the key factor that tumor cells evolve from normal cells.(5)Nine of the core genes are significantly related to the survival status of lung adenocarcinoma patients: LINC01266 has been used as biomarker for predicting lung cancer prognosis.CDK5R2 can be a potential lung cancer grading and staging biomarker.GPC2 has been used as a candidate immunotherapeutic target in Neuroblastoma.NPTX2 hypermethylation promotes cell proliferation but inhibits cell cycle arrest and apoptosis in gastric cancer cells by suppressing the p53 signaling pathway.GPC2 and NPTX2 need to be investigated in lung cancer.