Preparation And Pharmacodynamics Of Nanoparticle Hydrogel Of Insoluble Components Of Traditional Chinese (Mongolian) Medicine

Objective: This paper discussed a method of preparing nanoparticle hydrogel from insoluble components of Chinese(Mongolian)medicine and its potential mechanism and therapeutic effect on inflammation,hoping to find a solution to the low bioavailability of Mongolian medicine Menken Usu oral and easy to produce toxic accumulation through nanocomposite hydrogel technology.Methods:1)The mercury sulfide nanoparticle hydrogel was prepared by physical encapsulation method,and the maximum drug loading of the hydrogel was investigated.It was characterized by scanning electron microscopy(SEM),X-ray scattering techniques(XRD),and rotary rheometer,and its transdermal release in vitro was investigated.2)According to the relevant provisions of the 2020 edition of the Chinese Pharmacopoeia,the properties,particle size,stability and content of three batches of mercury sulfide nanoparticle hydrogels were determined,and the stability of mercury sulfide nanoparticle hydrogels in centrifugal,cold and hot environments was investigated.3)The ability of cell migration was detected by cell marking,the expression of CD80 and CD86 in cells was detected by flow cytometry,and NF-κB related pathway IκBα、 p-IκBα、NF-κB 、 p-NF-κB was detected by Western Blot(WB)to explore the effect of mercury sulfide nanoparticle on lipopolysaccharide(LPS)induced RAW264.7macrophages.4)The left ear swelling of the low,middle and high dose mercury sulfide nanoparticle hydrogel group was significantly lower than that of the model control group(p<0.05).In the rat toe swelling model,the right toe swelling of the middle and high dose mercury sulfide nanoparticle hydrogel group was significantly lower than that of the model control group(P<0.01).Pathological analysis showed that the infiltration details of lymphocytes in the fibrous tissue of the medium and high dose groups decreased,showing significant anti-inflammatory effects.Results:1)The hydrogel was successfully prepared,which showed an obvious porous structure.The pores were interconnected and evenly distributed,and the pore size range was about 20 um.In the range of 0.01～10 Hz,the gel will not be damaged due to the increase of frequency when the stress is constant,and the elasticity of the hydrogel will be enhanced after adding nanoparticle.The in vitro transdermal release conforms to the first-order release kinetics equation and has a sustained release effect.Compared with mercury sulfide nanoparticle,mercury sulfide nanoparticle hydrogel reduced skin penetration and skin retention.2)The quality control of the properties,p H value,particle size and mercury sulfide content of mercury sulfide nanoparticle hydrogel meet the requirements,and the stability is good in centrifugal,low temperature and high temperature environments.3)Mercury sulfide nanoparticle can restore the migration ability of RAW264.7 macrophages to a certain extent,can down regulate the expression of costimulatory molecule CD86 on the cell surface,and can also down regulate IκBα、p-IκBα expression.4)The left ear swelling of the low,medium and high dose mercury sulfide nanoparticle hydrogel group was significantly lower than that of the model control group(p<0.05).In the rat toe swelling model,the right toe swelling of the medium and high dose mercury sulfide nanoparticle hydrogel group was significantly lower than that of the model control group(P<0.01).Pathological analysis showed that the details of lymphocyte infiltration in the fibrous tissue of the medium and high dose groups were reduced,with anti-inflammatory effect.Conclusion: The nanoparticle hydrogel provided in this paper has simple preparation,sustained release effect,stable property,controllable quality,and can pass NF-κB pathway regulates LPS induced inflammatory response and exhibits inhibitory effects on both rat foot swelling model and mouse ear swelling model.Therefore,mercury sulfide nanoparticle hydrogel has the potential to significantly inhibit acute inflammation,which provides an experimental basis for the development of drugs to treat inflammation related diseases.