| Objective To investigate the expression of OCT-4 and CD133 in pancreatic cancer tissues and normal adjacent tissues.To analyze the correlation between OCT-4 and pancreatic cancer stem cells,the relationship between OCT-4 and clinicopathological characteristics of pancreatic cancer patients,and the role of OCT-4 in value-added metastasis.Method Buy pancreatic cancer tissue chip samples(serial number: HPan-Ade200Sur-02;Shanghai Core Ultrasound),the chip includes: survival pancreatic cancer 100 cases of cancer tissue and 80 cases of paracancerous tissue.The operation time was 2004.9 / 2008.12,and the follow-up time was 2011.12.The patients were followed up for 7 years.OCT-4 and CD133,a marker of pancreatic cancer stem cells,were detected by immunohistochemical method,and the relationship between their expression and pathological features in pancreatic cancer was analyzed.According to the expression of p53 and ki-67 in tissue microarray,the role of OCT-4 in value-added metastasis of pancreatic cancer was analyzed.Results 1.The positive expression of OCT-4 and OCT-4 staining was located in the cytoplasm and nucleus of pancreatic cancer,and brown and brown granules appeared in the positive sites.The expression rate of OCT-4 was 54% in pancreatic cancer tissues and 23.75% in adjacent normal tissues,and the expression level in pancreatic cancer tissues was significantly higher than that in adjacent normal tissues.2.The expression of CD133 was localized in the cell membrane and cytoplasm of pancreatic cancer by CD133 staining,mainly in the cell membrane and brown granules.The positive rate of CD133 was 51% in pancreatic cancer tissues and 20% in adjacent normal tissues.The level of CD133 in pancreatic cancer tissues was significantly higher than that in adjacent normal tissues(P < 0.01).3.The correlation between the expression of OCT-4 and CD133 in PC tissues was positively correlated with the expression of OCT-4 and CD133 in pancreatic cancer tissues.4.The relationship between OCT-4,CD133 and clinicopathological features of patients with PC the positive expression of OCT-4 in pancreatic carcinoma was not correlated with age,tumor location and tumor size.5.The relationship between OCT-4 and ki-67 and p53 in pancreatic carcinoma the positive expression of OCT-4 was negatively correlated with p53(r = 0.332,P < 0.0l),and positively correlated with ki-67(r = 0.374,P < 0.0l).6.Survival analysis showed that the survival time of OCT-4 negative expression group was longer than that of positive expression group,and the difference was statistically significant.Univariate or multivariate Cox regression analysis further confirmed that the positive expression of OCT-4 can be used as an independent risk factor for the prognosis of patients with pancreatic cancer.Conclusion 1.The expression of OCT-4 and CD133 in pancreatic cancer tissue suggests that there are pancreatic cancer stem cells in pancreatic cancer tissue,and OCT-4 may be a molecular marker of pancreatic cancer stem cells.2.The positive expressions of OCT-4 and CD133 in pancreatic cancer tissues suggest that the occurrence and development of PC is related to tumor stem cells,and the positive expression of OCT-4 may be involved in the occurrence and development of PC.3.There was a positive correlation between the expression of OCT-4 and CD133 in pancreatic cancer,suggesting that OCT-4 and CD133 had a synergistic effect in the occurrence and evolution of PC.The expression of OCT-4 and CD133 was not related to the age,pathological grade and tumor location of the patients.4.The expression of OCT-4 was negatively correlated with p53,while the expression of OCT-4 was positively correlated with ki-67.OCT-4 was involved in the proliferation and invasion of pancreatic cancer.5.The expression of OCT-4 can be an independent prognostic factor of pancreatic cancer,and the positive expression of OCT-4 will lead to low survival time and poor prognosis.OCT-4 is expected to become a new target for drug therapy of pancreatic cancer. |
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