| OBJECTIVE Breast cancer is one of the most common malignant tumors in women.Circulating tumor cells refer to cells that detach from the primary site of malignant tumor cells and enter the bloodstream through the blood vessels or lymphatic system.As the source of liquid biopsy,circulating tumor cells have broad prospects in clinical practice.In this study,the number was tested by im-FISH method that circulating tumor cells in the peripheral blood of breast cancer,and explored the significance of dynamic test of circulating tumor cells in the early diagnosis,assessment of therapeutic efficacy,promptly detection of the recurrent case,and prognosis prediction in breast cancer.METHODS 160 women who selected as the research objects were diagnosed as breast cancer at the Pathology Department from January 2018 to June 2021.The clinicopathologic data of patients eligible for inclusion were reviewed and collected.All patients were classified into hormone receptor positive group and negative group,based on the immunohistochemical expression of estrogen and progesterone receptor.6 ml of peripheral blood was collected before treatment and6 months,12 months,18 months after treatment,respectively.The CTC count was detected by im-FISH method,and the change of CTC count between hormone receptor positive group and negative group were analyzed.RESULTS Among the 160 breast cancer patients,there were 105 cases of hormone receptor positive patients.The positive rate of CTC was 52.38%(55/105)before treatment,29.52%(31/105)after 6 months treatment,26.67%(28/105)after12 months treatment,and 22.85%(24/105)after 18 months treatment.In 55 cases of hormone receptor negative breast cancer,the positive rate of CTC was 30.91%(17/55)before treatment,29.09%(16/55)after 6 months treatment,27.27%(15/55)after 12 months treatment,and 36.36%(20/55)after 18 months treatment.The difference in the positive rate of CTC between the hormone receptor positive group and negative group was statistically significant before treatment(χ~2=4.141,P=0.042),6 months after treatment(χ~2=25.120,P<0.001),12 months after treatment(χ~2=31.335,P<0.001),and 18months after treatment(χ~2=25.786,P<0.001).The positive rate of CTC before treatment was related to lymph node metastasis,vascular invasion and vascular tumor thrombus,and there was significant difference between the two groups(P<0.05),but not related to age and nerve invasion(P>0.05).Before treatment,the CTC count was 3(2,4)in hormone receptor positive group and 1(1,3)in hormone receptor negative group.The difference between the two groups was statistically significant(Z=18.645,P<0.05).The CTC count of breast cancer patients in hormone receptor positive group was 3(2,4)before treatment,2(1,3)6 months after treatment,1(1,3)12 months after treatment,and 1(1,2)18 months after treatment.The CTC count of hormone receptor positive breast cancer at 6 months,12 months and 18 months after treatment was statistically significant compared with that before treatment(χ~2=54.373,P<0.001).In this part,the CTC count before treatment compared with 6 months after treatment(Z=-5.522,P<0.001),12 months after treatment(Z=-4.911,P<0.001),and 18 months after treatment(Z=-5.576,P<0.001),the difference was also statistically significant(P<0.001).CONCLUSIONS1.The dynamic test of circulating tumor cell count in peripheral blood circulation has certain clinical application value in predicting the prognosis of breast cancer patients.2.The positive rate of circulating tumor cells in the peripheral blood is related to the clinicopathological characteristics of breast cancer,such as lymph node metastasis,vascular invasion and vascular tumor thrombus. |
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