Expression And Clinical Significance Of LncRNA CCAT1/microRNA-543 In Colorectal Cancer

【Objectives】By detecting colorectal cancer tissue and normal tissue adjacent to carcinoma of long chain noncoding RNACCAT1(Lnc RNACCAT1)and small RNA-543(mi R-543),analysis of Lnc RNA CCAT1 and mi R-543 expression in colorectal cancer tissue adjacent to carcinoma and whether there is a difference,and combining with clinical pathological parameters,explore the Lnc RNA CCAT1,mi R-543 in its role in the development of colorectal cancer and its clinical significance,for the treatment of colorectal cancer to provide a therapeutic targets or methods of early diagnosis.【Methods】1.Tissue samples: From June 2021 to June 2022,68 cases of cancer and paracancerous samples(> 5cm from the edge of the tumor)were collected from patients diagnosed with colorectal cancer in the Department of General Surgery,the First Affiliated Hospital of Dali University.2.Collection of clinicopathological information: The samples of this study were collected and their basic information was statistically analyzed,including smoking history,gender,tumor diameter,and tumor pathology,etc.3.The expression of long non-coding RNACCAT1(Lnc RNACCAT1)and micro RNA-543(mi R-543)in colorectal cancer and adjacent pathological tissues was detected by Quantitative reverse transcripton PCR(RT-q PCR).The correlation between Lnc RNACCAT1,mi R-543 and clinicopathological parameters of patients was analyzed.【Results】1.The expression of long non-coding RNA CCAT1 in colorectal cancer tissues was higher than that in adjacent normal tissues(P < 0.05).The relative expression of colorectal cancer tissues(mean ± standard deviation)was 213.924 ± 128.867,the median was 199.189,and the relative expression of adjacent normal tissues was 8.548 ±39.424.The median was 3.228.2.The expression of mi R-543 in colorectal cancer tissues was significantly higher than that in adjacent normal tissues(P < 0.05).The relative expression of colorectal cancer tissues(mean ± standard deviation)was 2.711 ± 1.434,the median was 3.086,and the relative expression of adjacent normal tissues(mean ± standard deviation)was0.851 ± 0.269.The median was 0.878.3.There was no significant difference in the expression level of mi R-543 in colorectal cancer patients with different gender,age,smoking history,CEA,T stage,tumor diameter and tumor pathological morphology.The relative expression of mi R-543 in colorectal cancer patients with lymph node metastasis N1 / 2 was significantly higher than that in colorectal cancer patients with lymph node metastasis N0(P = 0.007 < 0.05).4.There was no significant difference in the expression level of CCAT1 in colorectal cancer patients with different gender,age,smoking history,T stage,tumor diameter and tumor pathological morphology.The relative expression of CCAT1 in colorectal cancer patients with CEA > 5(ng / m L)was significantly higher than that in colorectal cancer patients with CEA ≤ 5(ng / m L),and the difference was statistically significant(P = 0.015 < 0.05).The relative expression of CCAT1 in colorectal cancer patients with lymph node metastasis N1 / 2 was significantly higher than that in colorectal cancer patients with lymph node metastasis N0,and the difference was statistically significant(P = 0.028 < 0.05).5.The correlation coefficient between mi R-543 and CCAT1 in colorectal cancer was 0.606,and showed a significant level of 0.01,indicating that there was a significant positive correlation between mi R-543 and CCAT1.【Conclusions】1.CCAT1 is highly expressed in colorectal cancer tissues,suggesting that CCAT1 may be involved in the occurrence of colorectal cancer.The expression of CCAT1 is associated with CEA and lymph node metastasis,and has certain specificity and sensitivity in the diagnosis of CRC.2.Mi R-543 is highly expressed in colorectal cancer tissues,suggesting that mi R-543 may be involved in the occurrence of colorectal cancer.Moreover,the expression of mi R-543 is associated with lymph node metastasis,and mi R-543 has certain value in the prognosis evaluation of colorectal cancer.3.CCAT1 may have a potential positive regulatory mechanism for mi R-543 and promote the occurrence of malignant colorectal tumors.