| Objective: mi RNAs are involved in the development of H-type hypertension.In this study,we investigated the association between the expression levels of mi R-21/-29/-199 related to homocysteine(Hcy)metabolism and H-type hypertension in Kazakhs in Xinjiang,and the effect of methylenetetrahydrofolate reductase MTHFR rs1801133 gene polymorphism,a key gene of Hcy metabolism,on the association of the above mi RNAs with H-type hypertension,and further analyze the combined effect of mi RNAs and the nutrient folate.To provide clues for studying the association of mi RNAs with H-type hypertension.Methods: A case-control study was conducted based on the data from a survey of the general population of Kazakhs in Xinjiang.The study subjects were divided into four groups: H-type hypertension group,simple hypertension group,simple hyperhomocysteinemia(HHcy)group,and normal control group.The group determined the MTHFR rs1801133 gene polymorphism and plasma folate concentration in advance,and the expression levels of mi R-21/-29/-199 in the samples were measured by q RT-PCR,respectively.M(Q1,Q3)was used to describe mi R-21/-29/-199 expression levels,and dichotomous unconditional logistic regression was used to analyze the association between mi RNA and H-type hypertension;and the effect of different genotypes of Hcy metabolism-related gene-MTHFR rs1801133 on the association between mi RNA and H-type hypertension was analyzed.Finally,the combined effect of mi RNA and folic acid on H-type hypertension was further analyzed.Results: 1.A total of 120 study subjects were included.The sex ratio(male/female)was 61/59 and the age was 45.48±8.60 years.The differences in the expression levels of mi R-21,mi R-29 and mi R-199 among the four groups of subjects were statistically significant(all P<0.05).mi R-21 was expressed less in the H-type hypertension group than in the control group,with M(Q1,Q3)of 0.19(0.07,0.60),0.78(0.45,1.23)respectively.mi R-29 was expressed higher in the H-type hypertension group than in the control group,with M(Q1,Q3)of 0.19(0.07,0.60),0.78(0.45,1.23)respectively.The expression of mi R-29 was higher in the H-type hypertension group than in the control group,with M(Q1,Q3)of 1.30(0.83,2.21),0.91(0.70,1.11),respectively.2.Taking the healthy group as control,up-regulation of mi R-21 expression level was a protective factor for H-type hypertension(OR=0.25,95%CI:(0.08,0.79));up-regulation of mi R-29 was a risk factor for H-type hypertension(OR=2.29,95%CI:(1.03,6.12);OR=3.20,95%CI:(1.04,9.88));mi R-199 was not associated with H-type hypertension(P=0.940).Taking hypertension alone as a control group,mi R-21 remained a protective factor for H-type hypertension(OR=0.40,95%CI:(0.20,0.79)).3.Taking the healthy group was used as control,no association of mi R-21/-29/-199 with H-type hypertension was found in either rs1801133 CC or CT+TT genotypes(all P>0.05).However,taking the hypertension-only group as a control,mi R-21 was a protective factor for H-type hypertension among hypertensive patients in the CT+TT genotype(OR=0.23,95% CI:(0.09,0.61)),and no such association was found in the CC genotype.4.The results of the combined effect analysis showed that the high levels of folate were protective factors for H-type hypertension,regardless of the mi R-21 expression level,when the healthy group was used as control(OR=0.03,95%CI:(0.00,0.36);OR=0.14,95%CI:(0.26,0.74)).Conclusions: 1.mi R-21 was up-regulated as a protective factor for H-type hypertension.mi R-29 was up-regulated as a risk factor for H-type hypertension.No association has been found between mi R-199 and H-type hypertension.In hypertensive patients with rs1801133 CT+TT genotype,mi R-21 up-regulation was a protective factor for H-type hypertension,no association has been found between mi R-29/-199 and H-type hypertension,and none of the above associations were found in CC genotype.2.The results of the combined effect analysis showed that the high levels of folate were protective factors for H-type hypertension,regardless of the mi R-21 expression level. |
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