Observation Study Of Hsa＿circ＿0023255 And Hsa＿circ＿0006391 In Type 2 Diabetes In Han Population

Objective: The expressions of hsa＿circ＿0023255 and hsa＿circ＿0006391 in the peripheral blood of the type 2diabetes mellitus group(T2DM group)and healthy control group(NC group)in Han nationality were compared.To analyze the correlation between hsa＿circ＿0023255,hsa＿circ＿0006391 and type 2 diabetes,and to provide ideas for the pathogenesis of type 2 diabetes.Methods: 1.According to the inclusion and exclusion criteria,20 Han patients with type 2 diabetes mellitus and 20 healthy controls were randomly selected.Basic data such as gender,age,BMI,SBP,DBP,and peripheral blood samples of all subjects were collected.2.An automatic biochemical analyzer detected the levels of FPG,2H-PG,TC,TG,LDL-C,and HDL-C.FINs and CP were detected by Roche electrochemical immunoassay analyzer.HOMA-IR and HOMA-β were calculated.The expression of hsa＿circ＿0023255 and hsa＿circ＿0006391 was detected by real-time fluorescence quantitative PCR.Bioinformatics analysis of the biological functions and enriched biological pathways of hsa＿circ＿0023255 and hsa＿circ＿0006391.Results: 1.There were no significant differences in gender,age,BMI,SBP,DBP,TG,LDL-C,and CP between the NC group and T2 DM group(P > 0.05).Compared FPG,2H-PG,TC,FINs,and HOMA-IR in the T2 DM group were higher than in the NC group L-C and HOMA-β were lower(P < 0.05).2.Compared with the NC group,the relative expression levels of hsa＿circ＿0023255 and hsa＿circ＿0006391 in the T2 DM group were higher than those in the NC group(P < 0.05).3.Correlation analysis showed that the relative expression levels of hsa＿circ＿0023255 and hsa＿circ＿0006391 were correlated with FPG,2H-PG,TC,FINs,and HOMA-IR,and HOMA-β(P < 0.05).4.Logistic regression analysis showed that the increased relative expression levels of hsa＿circ＿0023255 and hsa＿circ＿0006391 were risk factors for type 2 diabetes in the Han population.5.ROC curve showed that hsa＿circ＿0023255 and hsa＿circ＿0006391 had a good diagnostic value for type 2 diabetes in the Han population.6.GO functional analysis found that hsa＿circ＿0023255 and hsa＿circ＿0006391 may be involved in biological processes such as nuclear transcription or cell cycle.KEGG pathway analysis showed that hsa＿circ＿0023255 and hsa＿circ＿0006391 may be involved in the PI3K-Akt signaling pathway.Conclusion : 1.Compared with the healthy group,the expression levels of hsa＿circ＿0023255 and hsa＿circ＿0006391 were increased in the Han nationality patients with type 2 diabetes.2.hsa＿circ＿0023255and hsa＿circ＿0006391 have potential clinical diagnostic value for type 2 diabetes in Han population,and hsa＿circ＿0023255 has better clinical value for the early diagnosis of type 2 diabetes.