Research And Equipment Development Of In Vitro Culture Monitoring Technology For Living Organs Of Biological Manufacturing

Using stem cell technology to construct living tissues and organs is one of the ideal ways to solve the shortage of organ donors and build pathological models of diseases.It is an important link in the research of organ manufacturing to construct tissues and organs through biological 3D printing technology,and then conduct in vitro culture and monitoring of various physical and chemical indicators.In view of the characteristics of Gel MA hydrogel large hepatic tissue vascular network made by biological 3D printing technology,such as high transparency,poor mechanical performance,and difficulty in cultivating internal cells,this topic designs and builds a real-time monitoring platform for in vitro culture,so as to develop targeted in vitro culture monitoring technology,which can realize in vitro perfusion culture of hydrogel like hepatic tissue vascular network and real-time monitoring of culture status.The key contents include the selection of experimental equipment and the design and construction of the platform,the perfusion culture experiment of the vascular network,the development and verification of the flow control algorithm of the culture medium,the development and verification of the real-time monitoring algorithm of perfusion culture status,and the development of the supporting control software.First of all,according to the characteristics of the hydrogel mass vascular network itself and the indicators that need attention in the cultivation process,select the corresponding detection instruments,and design and build an in vitro perfusion culture real-time monitoring experimental platform.The platform needs to have the characteristics of high space utilization,convenient disassembly and sample replacement,and high degree of modularization,which provides a hardware basis for the perfusion monitoring experiment of the mass vascular network and the development and research of the corresponding technology.Secondly,through a large number of in vitro perfusion culture experiments of vascular network,the actual flow of culture medium in the perfusion process was measured,and appropriate flow calibration and control algorithms were developed.Then compare the perfusion culture experiments before and after applying the flow calibration control algorithm,and analyze and evaluate the impact of the flow calibration control algorithm on the perfusion effect of the culture medium under different flow rates.Then,during the perfusion culture,the industrial camera and the electronic display screen placed above the culture chamber are used to monitor and photograph the whole process of the culture state in real time,and an algorithm for image analysis and identification of the whole process of perfusion culture is developed,so as to realize the accurate identification of the damage of the massive vascular network and the penetration of nutrients during the culture process,and the whole process of perfusion culture will also be recorded and saved,It is helpful for us to analyze and study the relevant performance indicators of the large volume vascular network.Finally,the experimental verification test of each functional module of the experimental platform is carried out to evaluate the performance of the experimental platform.The results show that the experimental platform has the ability to calibrate the real-time flow of culture medium,monitor the cultivation status of large volume vascular network in real time,and detect the rupture of large volume vascular network in real time.