Study On Anti-hepatocellular Carcinoma And Its Mechanism Of Licoflavonol

Objective: This study investigated the antitumor effect of Licoflavonol in vitro and in vivo and the effect of Licoflavonol on autophagy and apoptosis of cells.The effect on proteasome was also discussed to provide theoretical basis for the research and development of its proprietary drugs and its application in the treatment of liver cancer.Methods: The cytotoxicity MTT test was used to detect the effect of Licoflavonol on the proliferation and activity of Hep G2,A549,ECA-109 cells.The anti-tumor effect of Licoflavonol was evaluated by tumor weight and the toxicity was evaluated by the change of mouse organ index.The effects of Licoflavonol on autophagy and apoptosis of Hep G2 cells were detected by flow cytometry,the effects of Licoflavonol on the expression of P53,Bcl-2,Bax,Beclin-1,and LC3 were analyzed by Western blot.Fluorescence substrate method was used to detect the effect of Licoflavonol on the activity of proteasome.Results:(1)The MTT assay results showed that Licoflavonol inhibited the proliferation of Hep G2,A549,and ECA-109 cells,ranging from 6.25 to 100 μM Within the range,the inhibitory effect gradually increases with the increase of the concentration of Licoflavonol.(2)The experimental results of tumor bearing mice showed that compared with the model group,there was a significant difference in the inhibition of tumor growth between the two dosage groups of Licoflavonol(6.25mg/kg and 25mg/kg).Licoflavonol has no effect on animal weight,heart,liver,spleen,and kidney.(3)The results of autophagy staining showed that Licoflavonol can induce autophagy in Hep G2 cells in a concentration and time dependent manner.(4)The results of cell apoptosis showed that with the extension of action time and the increase of Licoflavonol concentration,the apoptosis rate of cells gradually increased,with good concentration and time dependence.(5)Western blot results showed that the expression of Beclin-1,LC3 II,p53,Bax,and Bcl-2 in Hep G2 cells significantly increased with the prolongation of Licoflavonol action time and concentration.(6)The fluorescence substrate method detection results indicate that licorice flavonol has an inhibitory effect on two of the 26 S extracted from both intracellular and extracellular cells β Catalytic subunit caspase-like(β 1,caspase like),Chymotrypsin like(β 5,chymotrypsin like)has inhibitory effects and certain selectivity towards catalytic subunits.Conclusion: Licoflavonol could inhibit the growth of various tumor cells and was sensitive to Hep G2 cells.Licoflavonol can inhibit the growth of tumor cells in vivo,and has less toxicity than 5-FU.The anticancer mechanism may be related to promoting autophagy and inducing apoptosis.The fluorescence substrate experiment showed that Licoflavonol had the effect of inhibiting the activity of proteasome.