Study On The Anti-hypopharyngeal Squamous Cell Carcinoma By HOXA11-AS And Its Molecular Mechanism By The Chinese Medicine Monomer Solamargine

Objective: In this study,Solamargine(SM),the main active ingredient of traditional Chinese medicine Solanum nigrum,was selected to be applied to human hypopharyngeal squamous cell carcinoma Fa Du cells.To investigate the inhibition effect of SM on Fa Du cells,explore the regulatory process of SM on long non-coding RNA expression level and the molecular mechanism of its anti-Hypopharyngeal squamous cell carcinoma(HSCC)function,and provide suggestions for the development of new therapeutic agents for HSCC.Methods: In this experiment,we treated human hypopharyngeal squamous cell carcinoma Fa Du cells with SM.First,the effects of SM treated on Fa Du cell viability and cell proliferation ability were analyzed by MTT,Calcein AM /PI staining assay,and Ed U cell proliferation staining assay.Wound healing assay and Transwell assay were performed to detect the effects of SM on the migration ability and invasion ability of Fa Du cells.The impact of SM on the cell cycle and apoptosis of Fa Du cells was detected using flow cytometry.In addition,Lnc RNA sequencing was performed on Fa Du cells treated with SM.Based on the sequencing data,we combined bioinformatics analysis and Western Blot,RT-q PCR,and other assays to identify the Lnc RNAs that play a regulatory role and analyze the molecular mechanism of the SM effect on anti-HSCC by regulating Lnc RNA expression.Results: The results showed that SM significantly inhibited the viability and proliferation of Fa Du cells,as well as the migratory and invasive abilities of the Fa Du cells.High-throughput sequencing data showed that Lnc RNA HOXA11-AS was significantly down-regulated in Fa Du cells treated with SM,and regulated the expression of downstream mi R-155 and c-Myc.C-Myc is a proto-oncogene that affects cell proliferation,differentiation,as well as apoptosis,and its m RNA is considered to be the target gene of mi R-155.We found that the down-regulation of c-Myc expression by SM was accompanied by an increase in apoptosis,which was consistent with the results of transcriptome sequencing.Conclusion: Our study confirmed the inhibitory effect of SM on HSCC and observed that SM regulates mi R-155/c-Myc expression through the down-regulation of HOXA11-AS.The results reveal the molecular mechanism of SM anti-HSCC via HOXA11-AS.These results suggest that SM is a potential natural antitumor agent and provides theoretical support for its application in the therapy of HSCC.