Study On The Construction Of Vascularized Islet Like Organs And Its Therapeutic Effect On Type 1 Diabetes Mice

Up to 2022,there will be 8.75 million type 1 diabetes patients worldwide.Although China is one of the countries with the lowest incidence rate of T1 DM in the world,it has become a disaster area for diabetes,with the largest number of adult diabetes patients in the world,and the incidence of type I diabetes is increasing year by year.At present,there is no radical cure for diabetes.Patients can only delay their condition by taking oral hypoglycemic drugs or injecting insulin.Pancreas or islet transplantation can partially or completely restore physiological insulin secretion,but there are limitations of donor scarcity and immune rejection.Therefore,it is urgent to develop new strategies for type 1 diabetes.Pancreatic islet like organs can solve the problem of immune rejection caused by scarce pancreatic donors and organ transplantation,which is a research hotspot in the field of diabetes.Most scientists in this field use embryonic stem cells and induced pluripotent stem cells to induce differentiation into β Cells,and on this basis to build pancreatic islet organoids.Many studies and clinical trials at home and abroad have shown that mesenchymal stem cells may alleviate type 1 diabetes through immune regulation,repair and regeneration.Perinatal tissues(umbilical cord and placenta)are the main source of mesenchymal stem cells β.There are few studies on the therapeutic effect of mesenchymal stem cells inducing differentiation into pancreatic islet organoids and regulating the recovery of blood glucose in patients,and the relevant details are still blank.In this study,we intend to establish an umbilical cord derived mesenchymal stem cell line through the explant method,and induce it to differentiate into cells with insulin secretion function,and combine human umbilical vein endothelial cells and Col-Gelma materials to construct it as a vascularized pancreatic islet organoids.Verify its function in vitro through immunofluorescence and glucose stimulation,and establish a diabetes mouse model by streptozotocin induction.Through renal capsule transplantation,further explore the impact of vascular islet like organs constructed from encapsulate materials on the mouse model,to investigate the blood glucose reversal function of vascularized islet-like organs in mice.The results showed that Col-Gelma material could effectively promote the vascular branching of islet-like organs,and combined with histopathological HE staining and masson staining,it was found that the encapsulate material had no toxic side effects on mice;The results of graft recovery and immunofluorescence of mouse kidney sections showed that the packaging material could also promote the angiogenesis of pancreatic islets in vivo;At the same time,the blood sugar of the mice was recovered and the blood sugar clearance ability was partially improved.This study is useful for understanding the role of Col-Gelma in promoting the angiogenesis of pancreatic islet organoids in mesenchymal stem cell differentiation.The vascularized islet like organs developed by Col-Gelma in this paper will provide a new idea for understanding and treating diabetes.