Study On Flavonoids With Antioxidant Activity In Pericarp Of Tamarindus Indica Linn.

Object: This study focuses on identifying the chemical constituents,the antioxidant activity and angiogenesis inhibition activity of acetone extract from the pericarp of Tamarindus indica Linn(TIL).Method:(1)The acetone extract of the pericarp of TIL was separated and purified by various chromatographic materials.The structure of these compounds was identified according to the NMR data and others.(2)The antioxidant activities of acetone extract,dichloromethane extract,ethyl acetate extract,n-butanol extract and some flavonoids isolated from TIL were determined in vitro under the conditions of AAPH as substrate and Trolox as control.(3)To identify the compounds of antioxidant activity based on comprehensive analysis of the prediction data of network pharmacology,literature and the amount of substance of compounds obtained.The activity of inhibiting angiogenesis in vitro was tested by EA.hy926 cells.Results:(1)30 compounds were isolated and identified from the acetone extracts of the pericarp of TIL,in which 15 compounds(5、8、11、13、16、17-19、22、24、26-30)were isolated from the genus for the first time.(2)The results of ORAC experiment showed that 4 sorts of extracts had good antioxidant ability in vitro.11 flavonoids were determined,and the antioxidant activity of 9 flavonoids(eriodictyol,dihydrokaempferol,7,3’,4’-trihydroxylflavone,luteolin,naringin,catechin,kaempferol,quercetin,acacetin)were higher than Trolox.(3)The prediction results of network pharmacology showed that there were 14 targets of flavonoids to inhibit angiogenesis.The results of EA.hy926 cells experiment showed that 7-hydroxy-flavone and 7,4’-dihydroxyflavone decreased cell survival rate,7,3’,4’-trihydrox-yflavone,butin,apigenin,kaempferol,dihydrokaempferol increased cell survival rate.Conclusion: There are different types of flavonoids in pericarp of TIL.It mainly includes flavones,flavonones,flavonols,dihydroflavonols and flavanols.All of the extracts and 9 compounds of pericarp of TIL have good antioxidant activity in vitro.5 compounds showed the activity of promoting proliferation of EA.hy926 cell.