The Albizia Water Extract Promotes Neurogenesis And Reduction Of Depression-like Behavior In Depressed Mice

Major depression disorder(MDD)is a serious mental disorder with complex pathogenic factors.Clinical treatment is mainly drug therapy,but there is a lack of effective drugs with low side effects.Albizia julibrissin Durazz is a traditional Chinese medicine used to treat depression and anxiety but the mechanism is not clear.Adult hippocampal neurogenesis plays an important role in the treatment of depression,and we hypothesized that algaecia may achieve its antidepressant effect by promoting adult hippocampal neurogenesis.In this study,behavioral pharmacology was used to give oral administration of Albizia julibrissin Durazz Water Extract,AJWE to CD-1 socially frustrating depression model mice,and the efficacy of AJWE was similar to fluoxetine.AJWE significantly decreased the tail hanging time of mice,significantly increased the liking degree of sucrose,significantly increased the contact time with unfamiliar mice in the three-box social experiment,and significantly increased the time of depressed mice in the open field center.In this study,the effect of AJWE on adult hippocampal neurogenesis was detected by immunofluorescence method.The results showed that AJWE significantly increased the number of MCM2,DCX and GFAP positive cells in the hippocampal dentate gyrus of depressed mice,indicating that AJWE enhanced the proliferation and differentiation of hippocampal neural stem cells in mice.AJWE also significantly increased the number of MCM2 and BRDU co-labeled cells,suggesting that it may increase hippocampal neurogenesis in mice by promoting the entry of resting neural stem cells into cell division.Immunofluorescence method was used to detect the volume of hippocampal dentate gyrus in mice.The results showed that AJWE significantly increased the volume of hippocampal dentate gyrus in depressed mice.These results revealed that albizia alleviates depression-like behavior in mice by increasing hippocampal neurogenesis.This study provides a solid experimental basis for further development of albizia in the treatment of depression.